摘要
目的 测定大鼠星形胶质细胞 (astrocyte ,AST)培养液中雌激素的浓度。方法 取新生 2d大鼠大脑皮层进行AST原代及传代培养 ,分别于传代培养的第 0、7、14、2 1天进行细胞计数 ,同时ELISA方法测定培养液中雌二醇 (E2 )浓度。结果 细胞数量分别为 1× 10 4/ml、1.1× 10 6/ml、1.4× 10 6/ml、1.5× 10 6/ml;培养液中雌激素 (E2 )浓度分别为 (pg/ml) :0、( 117 0 3± 2 1 3 2 )、( 2 66 91± 2 2 0 3 )、( 2 5 2 62± 2 7 99)。第 0天 (换液当天 )培养液中未检测出雌激素 ,随着培养时间的延长雌激素浓度迅速增加 ,14d左右达到高峰 ,以后逐渐降低 ,但 2 1d时培养液中雌激素仍然保持较高的浓度。结论 体外培养新生大鼠大脑皮层AST可以分泌雌激素。
Objective To measure the estrogen concentration of estrogen (E 2) in the culture medium of rat astrocytes (ASTs). Methods Astrocytes in brain cortex of the 2-day-old neonatal rats were collected and cultured. The number of astrocytes was counted and the concentration of estrogen was measured by ELISA method at 0, 7, 14, and 21 d after culture. Results The cell counts were 1×10 4/ml, 1.1×10 6/ml, 1.4×10 6/ ml, and 1.5×10 6/ml, respectively. The concentrations of E 2 were: 0 pg/ml, (117.03±21.32) pg/ml, (266.91±22.03) pg/ml, and (252.62±27.99) pg/ml, respectively. No estrogen was detected in the primary culture medium. The concentration of estrogen increased in a time-dependent manner and reached the peak at 14 d, and then decreased gradually but remained at a certain level. Conclusion E 2 is secreted by astrocytes in the brain cortex of the 2-day-old neonatal rats.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2004年第19期1748-1750,共3页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目 ( 30 2 70 4 52 )~~
