摘要
目的 :建立牛黄复方栓剂中胆红素HPLC含量测定方法。方法 :采用C1 8反相柱 ,以二甲基亚砜 乙腈 0 5 %醋酸铵溶液 (1∶1 4∶1 )为流动相 ,4 5 6nm为检测波长 ,测定牛黄复方栓剂中胆红素的含量 ;供试品溶液制备采用二甲基亚砜 乙腈 (9∶4 )混合溶液超声提取。结果 :胆红素在 0 1 0 7μg～ 2 1 4 μg范围内与峰面积呈良好的线性关系 ,r =0 9995 (n =5 ) ;回收率为 1 0 1 0 %,RSD =0 5 1 8%(n =5 )。结论 :本法专属性强 ,简便快速 ,精密度良好。
Objective: To establish a method of determinating content of bilirubin in Niuhuang Compound Suppository by HPLC.Method: C_ 18 column was used.The mobile phase was consisted of dimethylsulfoxide:acetonitrile:0.5% acetic ammonium (1∶1.4 ∶1).Detector wavelength was 456 nm.The extraction solution for bilirubin was consisted of dimethylsulfoxide:acetonitrile= 9∶4 .Results: The linearity was obtained over the range of 0.107～2.14 μg. The mean recovery was 101.0%,RSD=0.518%(n=5).Conclusion:The method was sensitive,simple and accurate.
出处
《中药材》
CAS
CSCD
北大核心
2004年第10期772-774,共3页
Journal of Chinese Medicinal Materials
