摘要
目的 研究甲醛对小鼠胸腺淋巴细胞DNA的交联作用 ,探讨甲醛的遗传毒性作用及机制。方法 分别以γ射线及紫外线作为标准断裂剂 ,用单细胞凝胶电泳技术对不同浓度甲醛诱导的小鼠胸腺淋巴细胞DNA交联作用进行体内及体外实验研究。结果 体内和体外研究均显示 ,甲醛可以引起小鼠胸腺淋巴细胞DNA的交联作用 ;体外研究还发现 ,随着甲醛浓度的增加 ,交联作用加强 ,彗星细胞率分别为10 0 % ,76 % ,2 % ,平均尾长分别为 15 32 ,9 79,5 0 2 μm。结论 甲醛是一种DNA交联剂 ,具有诱导小鼠胸腺淋巴细胞DNA交联的作用。
Objective To explore the DNA cross-links in thymocytes of formaldehyde exposed mice and explore genotoxic effects of formaldehyde and possible mechanism.Methods γ-rays and UVL were used as standard DNA breaking agents,the DNA cross-links in thymocytes of mice induced by formaldehyde were studied in vitro and vivo with single cell gel electrophoresis assay (SCGE,comet assay).Results The consequence of the assay showed that formaldehyde could cause DNA cross-links of thymocytes of mice within the doses given,and cross-links were increased with the concentration of formaldehyde in vitro studies.Conclusions Formaldehyde was a DNA cross-links agent,which can cause DNA cross-links in thymocytes of mice,and there was a dose-dependent manner in vitro assay.
出处
《工业卫生与职业病》
CAS
CSCD
北大核心
2004年第6期360-363,共4页
Industrial Health and Occupational Diseases
基金
江苏省社会发展基金资助 (BS2 0 0 10 5 0 )
江苏省高校自然科学基金 ( 0 1KJB3 3 0 0 0 )
关键词
甲醛
小鼠胸腺淋巴细胞
DNA交联
单细胞凝胶电泳
Formaldehyde
Mice thymocytes
DNA cross-links
Single cell gel electrophoresis assay
