摘要
目的 研究垂体瘤转化基因 (PTTG)和 c- myc基因在骨髓增生异常综合征 (MDS)患者中的表达并分析其与 MDS的关系。方法 采用 RT- PCR方法检测 32例 MDS患者及 10例正常骨髓中 PTTG和 c- myc基因m RNA表达。结果 MDS患者 PTTG和 c- m yc基因表达明显高于对照组 (P<0 .0 5 ) ;PTTG和 c- myc基因在RAEB- 1及 RAEB- 2组中的表达高于 RA及 RAS(P<0 .0 5 ) ,RAEB- 1及 RAEB- 2组中的表达差异无显著意义(P>0 .0 5 )。结论 PTTG和 c- m yc基因的过表达可能与 MDS的发生发展有关。
Objective To explore the expression of PTTG and c-myc gene in myelodysplastic syndrome (MDS) and their relationship with MDS.Methods Expression of PTTG and c-myc gene mRNA was detected in BMMNC from 32 patients with MDS and 10 normal controls by reverse transcriptase-polymerase chain reation (RT-PCR) .Results Expression of PTTG and c-myc gene in BMMNC was significantly higher in MDS patients than in normal controls(P<0.05).Acording to FAB classification the expression of PTTG and c-myc gene in BMMNC was higher in type RAEB-1 and RAEB-2 than RA and RAS(P<0.05).The expression of PTTG and c-myc gene in type RAEB-1 and RAEB-2 showed no significance (P>0.05).Conclusion Overexpression of oncogene PTTG and c-myc may be related to the progression and genesis of MDS.
出处
《山东医药》
CAS
北大核心
2004年第31期8-9,共2页
Shandong Medical Journal
