摘要
目的建立高效液相色谱法同时测定维康胶囊中脂溶性维生素A(Vit A),D2(Vit D2),E(Vit E)含量的方法.方法采用Puritex C18(200 mm×4.6 mm,5μm);甲醇为流动相;流速为1.0 mL·min-1;检测波长为266nm;柱温为40℃.结果 Vit A,VitD2,Vit E的检测限分别为0.5,0.1,1.5ng,线性范围分别为0.41～103.50,0.14～35.60,2.00～500.00μg·mL-1,平均回收率分别为99.89%,99.55%,99.53%.结论该方法简单、快速、灵敏度高、重复性好.
OBJECTIVE: To establish a HPLC assay for determining Vit A, Vit D 2, Vit E in multivitamin and multimineral capsules. METHODS: A Puritex C18 column (200 mm x 4.6 mm, 5 μm) was selected as separation column at 40C. The mobile phase was methanol. The flow rate was 1.0 mL·min-1. The detection wavelength was at 266 nm. RESULTS: The detection limits of vitamin A, D2 and E were 0.5, 0.1 and 1.5 ng, respectively. The linear ranges were 0.41 - 103.50, 0.14- 35.60 and 2.00 - 500.00 μg·mL-1, respectively. The average recoveries were 99.89%, 99.55% and 99.53%, respectively. CONCLUSION: The method was simple, rapid, sensitive and producible.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2004年第10期784-785,共2页
Chinese Pharmaceutical Journal
关键词
高效液相色谱法
维生素
维康胶囊
Bioassay
Drug products
High performance liquid chromatography
Methanol
Separation
