摘要
重金属离子Cd^(2+)、Hg^(2+)和Pb^(2+)均能替代Ca^(3+),与家兔阑尾B淋巴细胞中21kD钙结合蛋白(CaBP_(21))结合,诱导其疏水区暴露,表现出相同的Phenyl-Sepharose疏水亲和层析特性;这些离子与Ca^(3+)一样,不改变CaBP_(21)在SDS-聚丙烯酰胺凝胶电泳(PAGE)中的迁移;在重金属离子存在下,CaBP_(21)在非变性碱性甘油系统PAGE中的迁移较Ca^(2+)存在时快,但比EGTA存在时慢,其迁移率的改变量与金属离子半径有关。
Heavy me al cations including Cd2+, Hg2 + and Pb2 + can substitute for Ca2 + to bind CaBP21, a novel 21 kD calcium-binding protein purified from rabbit appendix B lymphocytes, and then to induce the exposion of it's hydrophobic domain (s) .The migration of CaBP21 on SDS-polyacrylamide gel electrophoresis(PAGE)was the same in the presence of Ca2 + ,Cd2+Hg2 + ,Pb2 + and EGTA.On alkaline glycerol PAGE, CaBP21 migrated more slowly in the prosence of heavy metal cations then in the presence EGTA, but more quickly then in the presence Ca2+. The cation-induced changes in electrophofetic mobilities may be related to their ionic radii.
出处
《基础医学与临床》
CSCD
1993年第3期67-69,共3页
Basic and Clinical Medicine
关键词
钙结合蛋白
钙离子
重金属离子
Calcium-binding protein Ca2+ Heavy metal cation Heavy metal toxicity
