摘要
目的 探讨醒脑启智胶囊药物血清对PC12细胞缺氧损伤的影响。方法 采用血清药理学方法 ,体外培养PC12细胞 ,用Na2 S2 O4产生缺氧损伤 ,观察PC12细胞形态学、MTT法细胞活力、乳酸脱氢酶LDH活性的变化。结果 醒脑启智胶囊药物血清对PC12细胞的形态改变具有明显的保护作用 ,可增强细胞活力 ,降低LDH活性。结论 醒脑启智胶囊药物血清能明显减轻PC12细胞的缺氧损伤 ,并呈现一定的剂量依赖关系。
Objective To study the effect of durg serum with Xingnaoqizhi capsule on damaged PC12 cells due to xygen-deficiency.Methods By method of serum pharmacology,We fostered PC12 cells in vitro, and hurt them by oxygen-deficiency with the Na 2S 2O 4, to observe the changes of cells in morphology,cells, vitality in MTT and LDH vitality.Results Xingnaoqizhi capsule shows significantly protective function on morphological changes of PC12 cells ,and can significantly strengthen the cell vitality and lower the LDH activity.Conclusion The drug serum with Xingnaoqizhi capsule could significantly alleviate the oxygen-deficiency damage of PC12,which present the certain relation on the dosage.
出处
《中西医结合心脑血管病杂志》
2004年第11期651-652,共2页
Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
基金
河北省重点科研资助项目 (No .0 1 2 762 0 7D)
