摘要
以沙田柚上胚轴为对象,进行上胚轴不定芽发生影响因素研究。结果表明:沙田轴上胚轴不定芽发生率达90%以上的培养条件是:外植体切段形态学向上竖放于培养基表面,培养基激素组成为6BA3mg/L+NAA0 2mg/L,糖浓度5%,暗培养时间为10～14d。
The main factors influencing shoot regeneration from epicotyls of Citrus Crandis (L.) Osbeck cv.Shatian-Yu in vitro were studied in this paper. The results showed that the best way to obstain shoot formation over 90 percent were as follows: the medium with 6BA 3 mg/L+NAA 0.2 mg/L +5 %sugar was chosen, explants should be cultured in darkness for 10 to 14 days, and apical ends of explants should be planted in the surface of medium.
出处
《西南农业学报》
CSCD
2004年第5期639-641,共3页
Southwest China Journal of Agricultural Sciences
基金
广西自然科学基金(桂科回0342004)
广西农科院科技发展基金(合同号2003002)资助
关键词
沙田柚
上胚轴
不定芽
C. Grandis (L.) Osbeck
epicotyls
shoots regeneration
