摘要
为观察血红素氧合酶 1与冠心病患者冠状动脉病变的关系 ,将经冠状动脉造影最少有一支血管狭窄≥5 0 %的冠心病患者 136例纳入该研究 ,并选择冠状动脉造影正常的 4 0例患者作为对照组。按照病变处斑块的形态分为Ⅰ型病变组 5 3例、Ⅱ型病变组 70例和Ⅲ型病变组 13例。利用免疫组织化学方法对冠心病患者外周血单个核细胞中血红素氧合酶 1的表达进行定位分析 ,并通过计算机图像分析系统分析血红素氧合酶 1表达的程度及强度。通过Westernblot脉病变组血红素氧合酶 1表达明显高于冠状动脉正常组 (P <0 .0 1) ;Ⅰ型病变、Ⅱ型病变和Ⅲ病变组的血红素氧合酶 1表达水平存在明显差异 (P <0 .0 1) ,且随着病变程度加重 ,血红素氧合酶 1表达水平升高。推测血红素氧合酶
Aim To valuate the Association between heme oxygenase-1(HO-1)and angiographic morphology of coronary lesions in patients with coronary heart disease (CHD). Methods One hundred and thirty-six patients with coronary heart disease who underwent coronary angiography were selected in this study,and 40 patients with normal coronary artery were selected as control. According to angiographic morphology of coronary lesions,these patients were split into type I(smooth borders)group(n1=53),type Ⅱ(irregular borders)group(70)and type Ⅲ(long and irregular lesions)group(n3=13). The levels of HO-1 protein expression in monocyte and lymphocyte coming from patients with CHD were tested by immunohistochemistry and western blot. Computer picture analyzing system were also used to measure the levels of HO-1 protein expression. Results HO-1 protein is located in cell plasma; levels of HO-1 protein expression in patients with coronary heart disease were significantly higher than those of the controls(P<0.01); levels of HO-1 protein expression were the highest in type Ⅲ group,then type Ⅱ group,and at last the type I group(P<0.01). Conclusions There is a higher expression of HO-1 in patients with CHD,the levels of HO-1 protein are associated with angiographic morphology of coronary lesions,suspecting that HO-1 is associated with severity of coronary heart disease.
出处
《中国动脉硬化杂志》
CAS
CSCD
2004年第5期581-584,共4页
Chinese Journal of Arteriosclerosis
基金
广东省医学科学研究基金 (A2 0 0 44 14 )资助
