摘要
基于核酸对桑色素 铝 (Ⅲ )配合物的荧光增强作用 ,以桑色素 铝 (Ⅲ )为荧光探针 ,考察该探针与核酸的结合反应 ,建立了新的准确测定核酸的方法 ,并研究了该三元荧光体系的作用机理。在 pH 8 5时 ,fsDNA ,ctDNA ,smDNA和 yRNA的浓度与桑色素 铝 (Ⅲ )的荧光强度成线性关系 ,响应范围分别为 0 2 5～1 5 0 ,0 2 5～ 2 0 0 ,0 10～ 1 6 0和 0 2 5～ 2 0 0 μg·mL-1,检测限 (3σ/K)分别为 3,2 ,2和 3ng·mL-1。测定了合成样品 ,回收率 93 3%～ 10 7 9% ,相对标准偏差小于 3 6 %
A fluorescence-enhanced system was developed for the determination of nucleic acids by using morin-aluminum(Ⅲ) complex as a new fluorescent probe. In aqueous solution, morin-aluminum(Ⅲ) complex showed maximum excitation and emission (wavelengths) at 420.0 nm and 532.8 nm, respectively, and its fluorescence could be greatly enhanced in the presence of nucleic acids. Under optimal conditions, the calibration graph was linear over the range 0.25-1.50 μg·mL^(-1) for fish sperm DNA, 0.10-1.60 μg·mL^(-1) for salmon sperm DNA, 0.25-2.00 μg·mL^(-1) for calf thymus DNA and 0.25-2.00 μg·mL^(-1) for yeast RNA. The corresponding detection limits are 3 ng·mL^(-1), 2 ng·mL^(-1), 2 ng·mL^(-1) and 3 ng·mL^(-1), respectively. Applied for the determination of nucleic acids in synthetic samples, the relative standard deviation for five replicates is less than 3.6%, and the recovery ranges from 93.3% to 107.9%. Additionally, the interaction mechanism of morin-aluminum(Ⅲ) with nucleic acids is also discussed.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2004年第10期1230-1234,共5页
Spectroscopy and Spectral Analysis
基金
国家科技专项 (2 0 0 1BA80 4A2 6)
福建省科技攻关计划 (项目 2 0 0 2Y0 51 )
福建省自然科学基金 (D0 31 0 0 1 )资助
