摘要
目的:观察自体脾组织移植术后不同时相再生脾组织中生长相关蛋白(growthassociatedprotein43,GAP-43)、神经生长因子(nervegrowthfac-tor,NGF)、TrkAmRNA的表达,初步探讨自体脾组织移植中GAP-43神经再生与NGF,TrkA的关系。方法:健康Wistar大鼠144只,雌雄不限,体质量100～120g,随机分为手术组(GAP-43组、NGF组、TrkA组各36只)和对照组(36只),术后15,30,60,90,120,180d取两组脾组织,行原位杂交、RT-PCR及图像分析法测定GAP-43,NGF和TrkAmRNA。结果:脾组织移植手术后30d,自体移植脾组织中出现GAP-43,NGF,TrkAmRNA;手术后90d达到高峰;手术后120～180d,移植脾组织中GAP-43,NGF,TrkAmRNA逐渐接近正常脾组织。术后30～90d,3组中GAP-43,NGF,TrkAmRNA的含量均明显高于对照组(P<0.05或<0.01)。结论:GAP-43mRNA在自体移植脾组织中有表达,其表达规律与移植脾组织中神经纤维的再生规律相一致。伴随着GAP-43mRNA的增加,NGF,TrkAmRNA也表现出相应的表达规律,提示NGF和TrkA与脾组织神经再生关系密切。
AIM:To observe the expression of growth associated protein 43(GAP 43), nerve growth factor(NGF) and TrkA mRNA in regenerated splenic tissue at different phases after splenic autotransplantation,and explore the relationship of neural regeneration of GAP 43 with NGF and TrkA. METHODS:Total 144 healthy Wistar rats in either gender and with a body mass of 100 g to 200 g were randomly divided into two groups: operation groups(GAP 43 group,NGF group,and TrkA group with 36 rats in each group) and control group(36 rats).The splenic tissues in the two groups were taken on day 15,30,60,90,120 and 180 postoperatively,and the expressions of GAP 43,NGF and TrkA mRNA were detected by in situ hybrdization(ISH), reverse transcriptional PCR(RT PCR) and image analysis. RESULTS:GAP 43,NGF and TrkA mRNA appeared on day 30 postoperatively,reached its peak on day 90 and became almost normal on day 120 180 postoperatively.The contents of GAP 43,NGF and TrkA mRNA in the three groups were significantly higher than those of the control group on day 30-90 postoperatively(P< 0.05 or P< 0.01). CONCLUSION:The expression of GAP 43 mRNA has been found in the autotransplant splenic tissue,and the expression regularity is in accordance with the regularity of regeneration of nerve in the autotransplant splenic tissue.The expression of NGF and TrkA mRNA shows corresponding expression regularity with the increasing of GAP 43 mRNA,which suggests that NGF and TrkA have a close relationship with the neural regeneration of splenic tissue.
出处
《中国临床康复》
CSCD
2004年第32期7183-7185,i004,共4页
Chinese Journal of Clinical Rehabilitation
