摘要
目的 :探讨环氧化酶 -2 (Cox 2 )抑制剂Nimesulide(NIM )对人肺腺癌A 5 49细胞血管内皮生长因子 (VEGF)及血管生成素(Angs)基因表达的影响及意义。方法 :不同浓度 ( 2 5、5 0、10 0、2 0 0 μmol/L)NIM处理人肺腺癌A 5 49细胞 2 4、48、72h后 ,放射免疫法测定细胞培养上清液PGE2 水平 ,半定量RT PCR检测NIM处理人肺腺癌A 5 49细胞后48h其VEGF及Ang 1、Ang 2mRNA的表达水平。结果 :NIM处理A 5 49细胞后 ,细胞培养上清液PGE2 水平下降 ,呈浓度和时间依赖性 ;NIM处理A 5 49细胞后 48h ,VEGF、Ang 2mRNA水平下降 ,呈浓度依赖性 ;Ang 1mRNA水平则无显著改变。结论 :NIM可抑制Cox 2活性及下调VEGF、Ang 2基因表达 ,该作用可能是Cox
OBJECTIVE: To investigate the influence of Nimesulide (NIM), a selective cyclooxygenase 2 (Cox-2)inhibitor, on vascular endothelial growth factor (VEGF) and angiopoietins (Angs) gene expressions in human lung adenocarcinoma cancer cell line A549.METHODS:A549 cells were treated with NIM (25, 50, 100, 200 μmol/L) for 24 h,48 h and 72 h and the PGE 2 level in the cells cultured supernatant was quantitated by the radioimmunoassay. Expressions of VEGF, Ang-1 and Ang-2 mRNA in A549 cells treated with NIM for 48h were measured by the semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis.RESULTS:The PGE 2 level in the cells cultured supernatant decreased by NIM in a concentration and time dependent manner. Expressions of VEGF and Ang-2 mRNA but not Ang-1 mRNA significantly reduced in a concentration dependent manner.CONSLUSIONS:NIM can inhibit Cox-2 activity and down-regulate VEGF and Ang-2 gene expressions, which may be involved in cancer angiogenesis inhibition mechanisms of Cox-2 inhibitor.
出处
《肿瘤防治杂志》
CAS
2004年第10期1031-1034,共4页
China Journal of Cancer Prevention and Treatment
