摘要
目的 :建立眠而安滴丸 (酸枣仁 ,五味子等 )中五味子醇甲含量测定的方法。方法 :高效液相色谱法 ,hypersilC1 8分析柱 ,甲醇 水 (6 5∶35 )为流动相 ,流速 1mL·min-1 ,柱温为室温 ,检测波长 2 5 4nm。结果 :五味子醇甲回归方程Y =6 77836X - 372 4 3,r =0 .9999,线性范围 0 .3～ 1 .8μg ,平均回收率 1 0 0 .2 %,RSD 2 .9%。 结论 :该方法快速、准确 ,可作为制剂的含量测定方法。
AIM: To establish a method for determination of schizandrol A in Mian'er'an Dripping Pills (Semen Ziziphi Spinosae, Fructus Schisandrae, etc.). METHODS: HPLC was used with hypersil C 18 column (250mm×4.6mm; 5μm), methanol water (65∶35) as a mobile phase and detection wavelength at 254nm. The flow rate was 1.0mL·min -1 and at room temperature. RESULTS: The calibration curve was linear (r= 0.9999). The average recovery was 100.2% and RSD was 2.9%, respectively. CONCLUSION: The method is simple, quick and specific, and provides a quality control for the dripping pills.
出处
《中成药》
CAS
CSCD
北大核心
2004年第10期808-810,共3页
Chinese Traditional Patent Medicine
关键词
五味子
五味子醇甲
HPLC
Fructus Schisandrae
schizandrol A
RP HPLC
