摘要
目的 :研究丹参药材中丹酚酸类成分的固相萃取预富集方法。方法 :使用C 1 8固相萃取柱和液相色谱 离子阱多级质谱联机检测技术 ,通过固相萃取小柱预处理样品 ,电喷雾多级质谱负离子模式全扫描检测 ,考察影响回收率的 4个主要因素 ,即泵真空度、固相萃取小柱上样量、洗脱剂用量及洗脱速率 ,利用正交实验进行萃取条件的优化。结果 :最佳萃取条件为 :真空度 0 .0 2× 1 0 6Pa ,上样量 0 .75mL ,洗脱剂用量 1 5mL ,洗脱速率 1 .0mL·s-1 。结论 :本方法操作简单 ,准确可靠 ,可作为丹参中丹酚酸类成分的前处理方法。
AIM: To apply the solid phase extraction (SPE) on salvianolic acids in Radix Salvia Miltiorrhizae by means of high performance liquid chromatography with mass spectroscopic detector (HPLC MSn). METHODS: A C 18 solid phase column and negative ion electrospray ionization (ESI) mode were used by orthogonal design to optimize four factors which might affect recoveries of samples, which included vacuum tightness, sample size, amount of eluting agent and eluting power. RESULTS: The optimized results were as follows: vacuum tightness was 0.002×106 Pa, sample size was 0.75 mL, amount of eluting agent was 15mL and eluting power was 1.0mL·s -1 . CONCLUSION: The established method is simple, reliable and suitable for the usual quality control method of salviamolic acids in Radix Salvia Miltiorrhizae.
出处
《中成药》
CAS
CSCD
北大核心
2004年第10期840-843,共4页
Chinese Traditional Patent Medicine
基金
陕西省自然科学基金 (2 0 0 0H10 )
关键词
丹参
丹酚酸
固相萃取
HPLC-ESI-MS^n
Radix Salvia Miltiorrhizae
salvianolic acids
solid phase extraction
HPLC ESI MSn
