摘要
目的 测定银黄颗粒剂中绿原酸和黄芩苷的含量。方法 用 HPL C测定 ,色谱柱 :十八烷基硅烷基键合硅胶柱 4 .6 mm× 15 0 mm;流动相 :乙腈 - 1%冰醋酸 (1∶ 9)和甲醇 - 0 .1%磷酸 (1∶ 1) ;检测波长为 32 6 nm和 2 74 nm。结果 此方法具有样品处理简便、色谱分离度、重现性好。结论 本方法可作为银黄颗粒剂的质量标准检测方法 。
Objective To determine the content of chlorogenic acid and baicalin in Yinhuang Granules.Methods HPLC was used with ODS C_(18) column.Acetonitrile-1%acetic acid(1∶9)and methanol-0.1%orthophosphoric acid were used as mobile phases.The detection wavelength was set at 326 nm and 274 nm.Results The sample was easy to deal with and the chromatogram's separability was good with a good reproducibility.Conclusion These methods can be used to control the quality of YinhuangGranules.
出处
《现代中药研究与实践》
CAS
2004年第5期45-47,共3页
Research and Practice on Chinese Medicines
