摘要
目的:应用体外培养骨髓间充质细胞,在细胞和分子水平观察补骨合剂对细胞分化的影响,探讨补骨合剂治疗骨质疏松症的机制。方法:分离SD大鼠骨髓间充质细胞,通过形态学、碱性磷酸酶、细胞内骨钙素、转化生长因子β1基因表达等指标,对重组人骨形态发生蛋白2(rhBMP2)诱导下的骨髓间充质细胞向成骨细胞分化的程度进行探讨。结果:一定浓度的补骨合剂可促进分化中的骨髓间充质细胞分泌碱性磷酸酶和骨钙素,增强rhBMP2的活性,促进分化中的骨髓间充质细胞表达转化生长因子β1,补骨合剂的这种促进骨髓间充质细胞分化作用与密钙息基本相同。结论:补骨合剂不仅可以增强rhBMP2的活性,促进分化中的骨髓间充质细胞表达转化生长因子β,大量分泌Ⅰ型胶原,以利于钙盐沉积;还可以促进rhBMP2诱导的骨髓间充质细胞分泌碱性磷酸酶和骨钙素,促进骨髓间充质细胞向成骨细胞表型分化,从而促进钙、磷在骨表面沉积。
Objective:To observe the influence of sthenia skeleton herbs mixture (SSHM) on the differentiation of SD rat's marrow stromal cells culture in vitro and investigate the treatment mechanism of SSHM on osteoporosis. Methods:Marrow stromal cells were separated from the SD rat. Differentiation of the marrow stromal cells to the osteoblasts induced by rhBMP2 was observed with morphology,alkaline phosphatase specific activity,inner-cell osteocalcin, transforming growth factor β1 gene expression, etc. Results: Sthenia skeleton herbs mixture could hasten the cell excreting alkaline phosphatase and osteocalcin, reinforce the activity of rhBMP2 and facilitate the cell express transforming growth factor β1. These effects were the same as Miacalcic. Conclusion:One of the principle of treatment on osteoporosis by sthenia skeleton herbs mixture is that it can reinforce the activity of rhBMP2 and hasten the marrow stromal cell express β transforming growth factor 1 and to excreting collagen Ⅰ . Thus, it is beneficial for the deposition of calcium salts. It can also hasten the induced marrow stromal cell by rhBMP2 to excrete alkaline phosphatase and osteocalcin, which can accelerate the transforming of the cells into osteoblasts. Sequentially accelerate the deposition of calcium and phosphorus on the bone surface.
出处
《中国骨伤》
CAS
2004年第10期589-592,共4页
China Journal of Orthopaedics and Traumatology
基金
福建省卫生厅资助项目(2002-201)
