摘要
目的:探讨Survivin蛋白表达与肝细胞肝癌(HCC)临床病理特征相关性及临床意义。方法:应用链霉菌抗生物素-过氧化物酶免疫组织化学法(SP法),检测67例HCC中Survivin、血管内皮生长因子(VEGF)的蛋白表达。结果:67例肿瘤组织中35例Survivin呈阳性表达,阳性率为52.2%。肿瘤组织表达阳性率高于癌旁组织(P<0.05)。Survivin的表达与HCC肿瘤镜下门静脉癌栓发生密切相关(P<0.05)。VEGF阳性表达者为41例,阳性率为61.1%。VEGF的表达与HCC肿瘤大小、门静脉癌栓发生以及肿瘤TNM分期密切相关(P<0.05)。应用Kaplan-Meier法制作累积生存曲线并经Long-rank检验,结果显示Survivin表达阳性与阴性组患者术后累积生存率有显著性差异(P=0.0018)。结论:HCC中Survivin、VEGF蛋白表达上调,Survivin和VEGF均与HCC门静脉癌栓发生密切相关。HCC患者Survivin表达者预后不良,应密切随访。
Objective: To study the expression of Survivin and the clinical significance for patients with HCC. Methods: Expression of Survivin and VEGF in 67 HCC patients were assessed by immunohistochemistry staining. Results: The Survivin's expression was detected in 35/67 (52.2%). The expression rate of Survivin was higher in HCC group than that of the adjacent nontumorous liver and the normal liver groups (P<0.05). There was significant correlation between the expression of Survivin in HCC and the tumor thrombus in portal vein (P<0.05). VEGF expression rate was 61.1%. The positive rate of VEGF was correlated significantly with tumor size, portal vein tumor thrombus and TNM stage (P<0.05). By Kaplan-Meier Log-rank estimation, it was found the positive expression of Survivin was associated with poorer survival compared with the negative expression(P=0.001 8). Conclusions: Survivin and VEGF is up-regulated in HCC patients. The intrahepatic hematogenous metastasis is related to expression of Survivin and VEGF. Survivin is demonstrated as the prognostic factor for patients undergoing hepatic resection.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2004年第21期1201-1203,1208,共4页
Chinese Journal of Clinical Oncology
基金
山东省青年医学科研基金资助(编号:200334)
