期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

实用型低密度石英谐振式生物传感器阵列检测系统的构建

Construction of Detection System of Practical Low-density Quartz Oscillation Biosensor Array
下载PDF
导出
摘要 目的　构建实用型低密度石英谐振式生物传感器 (quartz oscillation biosensor,QOBS)阵列检测系统。方法　首先利用精细微加工法制作单个 QOBS,在此基础上先后构建了粘接式以及夹具式 2× 5型 QOBS阵列 ;并自行开发研制自激式振荡电路、PESA V2 .0版频率记录分析软件 ;将它们与计算机联用以构建 QOBS阵列检测系统。结果　成功地制造出了在气、液相中稳定振荡的单个 QOBS,构建的粘接式及夹具式 QOBS阵列各有其优缺点 ;自激式振荡电路有非常强的振荡能力 ,在液相中起振非常容易 ,其频率稳定度也可达实用要求 ;PESAV2 .0版频率记录分析软件具有自行设置各初始参数 ,实时记录频率变化、自动绘制频率变化趋势图及分析结果等多项功能。结论　成功地构建出 QOBS阵列检测系统 ,搭建起一个生物检测的技术平台 ,为临床上病原性微生物的检测以及多种疾病的早期诊断提供一种新的方法。 OBJECTIVETo construct detection system of practical low-density quartz oscillation biosensor (QOBS) array. METHODSTechnology of fine processing was firstly used to make the solo QOBS. Based on it, glued and clamped 2×5 style QOBS array was constructed successively. Also we developed the self-oscillating circuit and the PESA version 2.0 software applying to record and analyze frequency. So the detection system of QOBS array was constructed by combining all these components. RESULTSSolo QOBS that realized stable oscillation both in air and liquid phase was successfully constructed. Both glued and clamped style QOBS arrays had its advantages and disadvantages. And self-oscillating circuit that was developed by ourselves had strong vibromotive ability in liquid phase and its stability of frequency could also meet the request of experiment. Self-developed PESA version 2.0 software had many functions such as setting up primary parameters, recording change in frequency in real-time, plotting figure of frequency change automatically, and so on. Data would be transferred to computer automatically after the collection and analysis. CONCLUSIONSQOBS array detection system was successfully constructed. A technical platform was built for bio-detection and hence could provide a new method for the detection of pathogenic microorganism and early diagnosis for many diseases.
作者 府伟灵 陈鸣 蔡国儒 刘明华 张波 蒋天伦 陈庆海 俞凡
机构地区 第三军医大学西南医院 中国电子科技集团公司第
出处 《中华医院感染学杂志》 CAS CSCD 2004年第1期1-5,共5页 Chinese Journal of Nosocomiology
基金 国家"863"计划(2002AARZ2023) 国家"九五"科技攻关项目(96-A23-04-04) 国家自然科学基金(30270388) 军队"九五"课题(98MO94) 军队"十五"课题(01MA180 01LO065) 重庆市应用基础研究项目(2002-10-78) 重庆市科委风险投资基金(0499405)
关键词 石英 传感器 核酸 Quartz Sensors Nucleic acid
分类号 R446.1 [医药卫生—诊断学]
  • 相关文献

参考文献15

  • 1[1]Vaezi-Nejad SM. A random walk in sensors: recent advances and market opportunities[J]. Measurement Control, 1998, 31(1): 80-85.
  • 2[2]Forster AH, Krihak M, Swanson PD, et al. A laminated, flex structure for electronic transport and hybridization of DNA[J]. Biosens Bioelectron, 2001, 16(3): 187-194.
  • 3[3]Boon EM, Ceres DM, Drummond TG, et al. Mutation detection by electrocatalysis at DNA-modified electrodes[J]. Nature Biotechnol, 2000, 18(10): 1096-1100.
  • 4[4]Heaton RJ, Peterson AW, Georgiadis RM. Electrostatic surface plasmon resonance: direct electric field-induced hybridization and denaturation in monolayer nucleic acid films and label-free discrimination of base mismatches[J]. Proc Natl Acad Sci USA, 2001, 98(7): 3701-3704.
  • 5[5]Jenison R, Yang S, Haeberli A, et al. Interference-based detection of nucleic acid targets on optically coated silicon[J]. Nature Biotechnol, 2001, 19(1): 62-65.
  • 6[6]Nakatani K, Sando S, Saito I. Scanning of guanine-guanine mismatches in DNA by synthetic ligands using surface plasmon resonance[J]. Nature Biotechnol, 2001, 19(1): 51-55.
  • 7[7]Matsuno H, Niikura K, Okahata Y. Design and characterization of asparagine and lysine-containing alanine-based helical peptides that bind selectively to A.T base pairs of oligonucleotides immobilized on a 27 mHz quartz crystal microbalance[J]. Biochemistry, 2001, 40(12): 3615-3622.
  • 8[8]Zhou XC, Huang LQ, Li SF. Microgravimetric DNA sensor based on quartz crystal microbalance: comparison of oligonucleotide immobilization methods and the application in genetic diagnosis[J]. Biosens Bioelectron, 2001, 16(1-2): 85-95.
  • 9[9]Towery RB, Fawcett NC, Zhang P, et al. Genomic DNA hybridizes with the same rate constant on the QCM biosensor as in homogeneous solution[J]. Biosens Bioelectron, 2001, 16(1-2): 1-8.
  • 10陈鸣,府伟灵,蔡国儒,刘明华,张波,蒋天伦,陈庆海,秦廷辉.肽核酸生物传感器直接检测临床标本中提取的病毒基因组DNA[J].中华医院感染学杂志,2003,13(7):616-619. 被引量：13

二级参考文献28

  • 1董献堆,陆君涛,查全性.巯基化合物自组装单分子层的研究进展[J].电化学,1995,1(3):248-258. 被引量：25
  • 2邓文礼,杨大本,方晔,白春礼.硫醇在Au(111)上SA单分子层膜研究[J].中国科学（B辑）,1996,26(2):174-180. 被引量：16
  • 3刘明华.[D].第三军医大学,2001:44-49.
  • 4裴仁军,胡继明,胡毅,曾云鹗.压电生物传感器研究进展[J].武汉大学学报（自然科学版）,1997,43(2):265-270. 被引量：2
  • 5Zhou XC, Huang LQ, Li SF. Microgravimetric DNA sensor based on quartz crystal microbalance: comparison of oligonu-cleotide immobilization methods and the application in genetic diagnosis[J]. Biosens Bioelectron, 2001, 16(1-2) : 85-95.
  • 6Nielsen PE, Egholm M, Berg RH, et al. Sequence-selective recognition of DNA by strand displacement with a thyminesubstituted polyamide[J]. Science, 1991, 254: 1497-1500.
  • 7Wittung P, Nielsen PE, Buchardt O, et al. DNA-like double helix formed by peptide nucleic acid[J]. Nature, 1994, 368(7) : 561-563.
  • 8Jensen KK, Orum H, Nielsen PE, et al. Kinetics for hybridization of peptide nucleic acids (PNA) with DNA and RNA studied with the BIAcore technique [J]. Biochemistry,1997, 36: 5072-5077.
  • 9Egholm M, Christensen L, Dueholm KL, et al. Efficient pH-independent sequence-specific DNA binding by pseudoisocy-rosin-containing bis-PNA[J]. Nucleic Acids Res, 1995, 23(2): 217-222.
  • 10Demidov VV, Yavnilovich MV, Belotserkovskii BP, et al.Kinetics and mechanism of PNA binding to duplex DNA[J].Proc Natl Acad Sci USA, 1995, 92: 2637-2641.

共引文献66

中华医院感染学杂志
2004年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部