摘要
目的 建立并评价一种快速原位诊断中心静脉导管相关性血行感染 (CRBSI)的方法。方法 应用革兰 -吖啶橙白细胞染色检测法检测 115例疑为中心静脉 CRBSI的患者 ,与常规培养方法相比较 ,评价其敏感性、特异性及可行性。结果 115例中有 4 3例 (37% )被革兰 -吖啶橙白细胞染色检测法诊断为中心静脉 CRBSI,以常规方法作参照 ,敏感性、特异性、阳性预测值、阴性预测值及准确度分别为 95 %、93%、88%、97%、94 % ,在对革兰阳性菌、革兰阴性菌和真菌鉴别诊断中 ,亦获得了较高的敏感性和特异性。结论 革兰 -吖啶橙白细胞染色检测法可作为原位诊断中心静脉 CRBSI的简单、实用的方法 ,有助于早期诊断及指导针对性应用抗生素。
OBJECTIVETo establish and evaluate an in situ method for the rapid diagnosis of central-venous-catheter-related bloodstream infection (CRBSI). METHODS The Gram-acridine-orange leucocyte stain test was used in 115 suspected cases of CRBSI, and its sensitivity, specificity and feasibility were compared with routine cultural method. RESULTSForty three cases (37%) were diagnosed as CRBSI by the Gram-acridine-orange leucocyte stain test. The sensitivity of the test was 95%, while 93%, 94%, 88%, 97% for the specificity, accuracy, positive and negative predictive value, respectively. And it also had relatively high sensitivity and specificity on the identification of G+, G-bacteria and fungi. CONCLUSIONS The Gram-acridine-orange leucocyte stain test is a simple, quick, economical and readily available method in the early diagnosis of CRBSI without catheter removal, and may facilitate treatment with early targeted antimicrobials.
出处
《中华医院感染学杂志》
CAS
CSCD
2004年第1期113-115,共3页
Chinese Journal of Nosocomiology
关键词
中心静脉导管
感染
诊断
Central venous catheter
Infection
Diagnosis
