摘要
对加拿大披碱草与野大麦2个四倍体亲本杂交产生的三倍体(2n=3x=21)属间杂种F1的自然加倍植株、自然加倍植株F1代及秋水仙素诱导加倍植株的染色体数目及配对构型、花粉育性、结实性进行了鉴定。结果显示:自然加倍植株、自然加倍植株F1代和秋水仙素诱导加倍植株的RTC染色体数目均为42条,即为六倍体(2n=6x=42);其PMCMⅠ平均染色体配对构型分别为0.04Ⅰ+20.96Ⅱ、0.03Ⅰ+20.98Ⅱ和0.03Ⅰ+20.97Ⅱ,配对行为规则;它们的花粉可育率分别为84.97%、94.08%和90.21%,自然结实率分别为77.51%、83.87%和80.13%。证明该属间杂种F1染色体已加倍,且染色体加倍植株的育性已得到恢复。
The chromosome number and pairing configuration,pollen fertility,seed set of the natural chromosome doubling plant,chromosome_doubling F_1 and colchicines induced chromosome doubling plant of triploid (2n=3x=21) intergeneric hybrid F_1 between Elymus canadensis and Hordeum brevisubulatum were identified. The results showed that the chromosome numbers in root tip cell of the natural chromosome doubling plant, natural chromosome-doubling F_1 and cochicines induced chromosome doubling plant of triploid hybrid F_1 are 42, which are hexaploids (2n=4x=42);their average chromosome pairing configuration at PMCMI are 0.04Ⅰ+20.96Ⅱ,0.03Ⅰ+20.98Ⅱ and (0.03Ⅰ)+20.97Ⅱ respectively, the chromosome pairing behavior is regular; their pollen fertility are 84.97%, 94.08% and 90.21% respectively,the seed set are (77.51)%,83.87%and 80.13% respectively.It was proved that the chromosome of intergenus hybrid F_1 had been doubled,and the fertility of chromosome doubling plant had been restored.
出处
《中国草地》
CSCD
2004年第5期1-8,共8页
Grassland of China
基金
国家自然科学基金项目(30160058)
内蒙古自然科学基金项目(2002305)
教育部留学回国人员基金项目(2001345)资助~~
关键词
加拿大披碱草
野大麦
属间杂种F1
染色体加倍植株
育性
Elymus canadensis
Hordeum brevisubulatum
Intergeneric hybrid F_1
Chromosome doubling plant
Fertility
