摘要
以实时荧光PCR技术鉴定商业化种植的转基因玉米T14/T25品系。根据转基因玉米T14/T25转入的外源基因质粒图谱,设计转基因引物和探针进行PCR和实时荧光PCR检测,建立了转基因玉米品系鉴定的实时荧光PCR方法。实验结果表明,用TaqMan探针可检测到T14/T25产生的荧光信号,而对其他玉米品系则检测不到荧光信号,为转基因产品的鉴定检测提供了新方法。
To identify genetically modified (GM) maize T14/T25 lines,a real-time fluorescent PCR (RTF PCR) assay was performed in this study. Primers and Taq man probes specific for inserted genes in the T14/T25 were used to conduct the real-time fluorenscent (RTF) PCR and PCR assays.The RTF PCR method was established to detect and identify GM maize lines.The results show that the Taq Man probe could identify T14/T25 maize used,while other GM and NO-GM maize didn't be detected.The RTF PCR could be a new method for detecting other genetically modified organism.
出处
《遗传》
CAS
CSCD
北大核心
2004年第5期689-694,共6页
Hereditas（Beijing)
基金
科学技术部国家转基因植物研究与产业化专项课题资助(课题类别及编号:J00 C 005)~~
