摘要
灰树花是一种珍贵的药用真菌,因为多糖含量较高,较难获得高质量的总DNA,本文提出了一种制备高质量灰树花总DNA及构建灰树花基因组文库的方法。该方法制备的灰树花总DNA,经Sau3AⅠ酶切后,用于构建基因组文库,可得到2×105个转化子/50mg,平均插入片段为14kb。为下一步克隆灰树花中的基因以及进行其他分子生物学研究奠定了基础。
Grifola frondosa, is a valuable medicinal fungus. High quality total genomic DNA is difficult to prepare due to its high polysaccharide content. A method for the preparation of Grifola frondosa total genomic DNA and construction of Grifola frondosa, genomic library is described. Genomic DNA prepared by this method is digested by Sau3A I restriction enzyme. Constructed genomic library give a titer of 2×10~5 transformants/50mg , with a average insert size of 14kb. This has paved way for the cloning of other Grifola frondosa genes and molecular biology studies.
出处
《遗传》
CAS
CSCD
北大核心
2004年第5期711-713,共3页
Hereditas（Beijing)
基金
广州市科委重大科技攻关资助项目(2001-Z-006-01)~~
关键词
灰树花
DNA提取
基因组文库
Grifola frondosa
DNA preparation
genomic library
