摘要
目的 考察可溶性鸡Ⅱ型胶原 (SCCⅡ )对大鼠胶原性关节炎 (CIA)的免疫治疗作用及机制。方法 检测CIA大鼠血清抗Ⅱ型胶原 (CⅡ )抗体、腹腔巨噬细胞 (PMΦ)与滑膜细胞产生白介素 1(IL 1)和肿瘤坏死因子α(TNFα)和SCCⅡ /刀豆球蛋白A(ConA)诱导 5个部位淋巴细胞的增殖反应 ,同时测量继发炎症反应和免疫器官系数。结果 ig 0 .0 3,0 .3和 3.0mg·kg- 1·d- 1SCCⅡ (d 14～ 2 2 ) ,明显减轻患鼠继发炎症 ,抑制针对SCCⅡ的皮肤迟发型超敏反应和PMΦ,滑膜细胞超常分泌IL 1和TNFα ,促进体重和免疫器官系数恢复 ,提升低下的淋巴细胞ConA反应 ,但不能降低血清抗CⅡ抗体水平 ,5个部位的淋巴细胞对SCCⅡ体外刺激也几无反应性。结论 SCCⅡ通过T细胞免疫耐受途径对大鼠CIA产生治疗作用。
AIM To investigate the immunotherapeutic eff ec ts of ig administration of soluble chicken collagen typeⅡ(SCCⅡ) on rat collage n induced arthritis (CIA) and its mechanism. METHODS Each male Wistar rat was immunized with 0.1 mL of the emulsion containing SCCⅡ(final con centration of 2 g·L -1) by intradermal injection(id) at the right hind paw on d 0, boosted one time on d 7 with 0.1 mL of the emulsion by id in the tail. The anti-typeⅡ collagen antibodies in sera, interleukin-1(IL-1) and tumor ne crosis factor α (TNFα) levels in the supernatant of peritoneal macrophages (PM Φ) and synovial membrane cells, and the concanavalin A(ConA)-induced prolife rative response of the thymocytes, splenocytes,draining lymph node cells, mesenteric lymph node cells and Peyer′s patch cells in CIA rats were assayed, and the measurements of the ankle joint secondary in flammatory response and the immune organ-indices in CIA rats were also take n. RESULTS Administration of 0.03, 0.3 and 3.0 mg·kg -1· d -1 SCCⅡ ig (d 14-22) could markedly ameliorate the secondar y inflammatory response, inhibit the delayed type hypersensitivity to SCCⅡ, dep ressed the IL-1 and TNFα production in PM Φ and synovial membrane cells, pro mote the recovery of the body weight and the immune organ-indices, improve the overreduced ConA-induced lymphocyte proliferative response, but could not decre ase the anti-CⅡ antibody levels. Furthermore, lymphocytes from 5 lymphatic tis sues were all nearly unresponsive to SCCⅡ. CONCLUSION ig administr ation of SCCⅡ may have immunotherapeutic effect on rat CIA via T lymphocytes im munotolerance path.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2004年第5期365-370,共6页
Chinese Journal of Pharmacology and Toxicology
关键词
可溶性鸡Ⅱ型胶原
免疫耐受
关节炎
免疫学
soluble chiken collagen typeⅡ
immune tolerance
arth ritis
immunology
