摘要
目的 考察单硬脂酸甘油酯固体脂质纳米粒 (monostearinsolidlipidnanoparticles ,MSLN)经PEG2 0 0 0修饰后 ,对A5 4 9细胞摄取MSLN及J774A1细胞吞噬MSLN的影响。方法 采用溶剂扩散法制备MSLN ,测定其粒径和zeta电位 ;以罗丹明B(RhodamineB)为荧光标记物 ,研究A5 4 9细胞对MSLN的摄取作用和J774A1细胞对MSLN的吞噬作用。结果 MSLN的细胞毒性较低 ,A5 4 9细胞对MSLN的摄取可快速接近饱和 ,其摄取百分率与MSLN在细胞外的浓度呈负相关。结论 MSLN经PEG2 0 0 0修饰 ,可显著抑制J774A1细胞对MSLN的吞噬 ,但可增加A5 4 9细胞对MSLN的摄取。
Aim To investigate the cellular uptake of monostearin solid lipid nanoparticles (MSLN) and the influence on the cellular uptake by MSLN modified with PEG2000 in human-type II cell alveolar epithelial cell line (A549) and murine macrophages cell line (J774A1). Methods MSLN were prepared by a novel solvent diffusion method. The particle size distribution and zeta potential of MSLN, measured by light scattering and electrophoretic mobility, were investigated. The cytotoxicity of MSLN and MTX-loaded MSLN in A549 cells were performed by the MTT method. Rhodamine B was incorporated into solid lipid nanoparticles as fluorescent marker, after PEG2000 integrating monostearin during preparation, the cellular uptake of MSLN by A549 and J774A1 cell lines were determined spectrofluorimetrically. Results The IC 50 of MSLN and MTX-loaded MSLN on A549 cells were 227.56 μg·mL -1 and 71.37 μg·mL -1, respectively. The percentage of cellular uptake showed a negative correlation to the concentration of MSLN in incubation medium and the internalization behaved rapidly. Contrary to situation in J774A1 cell line, internalization of solid lipid nanoparticles was promoted with increasing the content of PEG2000 incorporated into MSLN in A549 cell line. Conclusion After modifying MSLN with PEG2000, it represents relative lower phagocytosis by J774A1 cell line and higher uptake in A549 cell line.
出处
《药学学报》
CAS
CSCD
北大核心
2004年第11期876-880,共5页
Acta Pharmaceutica Sinica
基金
浙江省自然科学基金项目资助 (M3 0 3 817) .
关键词
固体脂质纳米粒
A549人Ⅱ型肺上皮细胞
J774A1巨噬细胞
细胞摄取
细胞吞噬
solid lipid nanoparticles
human-type II cell alveolar epithelial cell line A549
murine macrophages cell line J774A1
cellular uptake
phagocytosis
