摘要
目的 以三甲基-β-环糊精为手性选择剂,研究甲磺酸罗哌卡因对映体拆分方法。方法 用区带毛细管电泳模式。探讨三甲基-β-环糊精对甲磺酸罗哌卡因对映体的拆分的影响,确定拆分甲磺酸罗哌卡因对映体的最佳条件,并与β-环糊精、羟丙基-β-环糊精进行了对比。结果 以β-环糊精和羟丙基-β-环糊精作为手性选择剂时,不能使甲磺酸罗哌卡因对映体达到基线分离,而三甲基-β-环糊精能获得很好的分离结果。结论 本方法简便、快速,能比较准确地测定甲磺酸罗哌卡因对映体的纯度。
OBJECTIVE: To investigate the enantioseparation of ropivacaine mesylate using capillary electrophorsis (CE) with three cyclodextrins as the chiral additives. METHODS: The enantioseparation of ropivacaine mesylate was reported with β-CD, HP-β-CD and TM-β-CD as chiral selectors employing an uncoated capillary. The effects of the pH, composition and concentration of the running buffer solution, the kind and concentration of chiral selectors, the running voltage, the capillary temperature on the mobility and chiral separation were studied. RESULTS: TM-β-CD showed a baseline enantiomeric separation for ropivacaine, whereas the β-CD and HP-β-CD, under optimal conditions, was poor in stereoselectivity and showed just partial chiral separation. CONCLUSION; The enantioseparation is an accurate, reliable and convenient method for measuring enantiomeric purity of ropivacaine mesylate.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2004年第11期856-858,共3页
Chinese Pharmaceutical Journal
关键词
毛细管区带电泳
对映体分离
甲磺酸罗哌卡因
Capillarity
Electric potential
Electrophoresis
Oligomers
pH effects
Separation
Stereochemistry
