摘要
目的 探讨联合检测肿瘤标记物端粒酶、神经元特异性烯醇化酶 (NSE)对恶性胸腔积液的诊断价值。方法 用聚合酶链反应 酶联免疫吸附分析法 (PCR ELISA)检测胸腔积液端粒酶活性、用放射免疫分析法测定胸腔积液NSE水平 ,共检测了 6 6例恶性胸腔积液和 6 0例非恶性胸腔积液。结果 恶性胸腔积液组的胸腔积液端粒酶和NSE的阳性率分别为 80 .3%和 6 5 .2 %。非恶性胸腔积液的端粒酶和NSE的假阳性率分别为 5 %和 3.3%。联合检测胸腔积液端粒酶和NSE敏感性下降至 6 2 .1% ,但特异性上升至 98.3%。结论 检测胸腔积液端粒酶和NSE对癌性胸腔积液的诊断均有一定的价值 ,联合检测综合诊断更能提高诊断准确率。
Objective To explore the diagnostice value of telome rase assay combined with neuron specific enolase(NSE) in malignant pleural effusion. Methods Telomerase activity and NSE from 66 malignant pleural effusion cases and 60 non-malignant pleural effusion cases were detected by polymerase chain reaction-enzyme linked immunosorbent assay(PCR-ELISA) and radial immunosorbent assay(RIA). Results The positive rates of telomerase and NSE in the malignant pleural effusion patients were 80.3% and 65.2%, respectively. The false-positive rates of telomerase and NSE in the non-malignant pleural effusion patients were 5% and 3.3%, respectively. Conclusion The assays of telomerase and NSE of pleural fluidis are valuable to the diagnosis of malignant pleural effusion. The combination of the two assays increases the accuracy of the diagnosis.
出处
《临床荟萃》
CAS
北大核心
2004年第23期1336-1337,共2页
Clinical Focus
