摘要
目的阐明不同程度及不同时间脑挫伤后NF-κB表达的变化。方法应用免疫组织化学染色、Westernblot和RT-PCR方法对48例不同程度实验性脑挫伤大鼠的脑组织进行了检验,同时以3例非脑挫伤和3例假手术大鼠的脑组织做对照。结果伤后1hNF-κB即有表达、24～48h达高峰,一周后停止表达。阳性细胞多集中于挫伤区及周围带皮质和脑实质内海马区;不同程度脑挫伤组之间于伤后各个时间段NF-κB表达无明显差异。结论本实验首次通过对不同程度脑损伤后NF-κB表达量的研究,找到其表达的时间性变化规律,对确定脑挫伤形成时间有意义,但不能作为判断脑挫伤程度的指标。
Objective To investigate the expression of NF-κB in different post-traumatic intervals and severity of brain injury. Methods Fifty-four brain tissue samples of slight (n=24), moderate(n=24) brain injury, sham(n=3) and control (n=3) of rat were examined by immunohistochemical staining, westernblot and RT-PCR. Results Up-regulating of NF-κB expression was found in tissues from traumatic brain injury compared with controls in early 1 hour after TBI, and reached peak at 24h and 48h and disappeared after 7 days. The expression of NF-κB mRNA has association with contusion severity. Conclusion NF-κB may benefit to the estimation of posttraumatic intervals of brain injury.
出处
《法医学杂志》
CAS
CSCD
2004年第2期73-76,80,i010,共6页
Journal of Forensic Medicine
