摘要
目的:探讨Bcl-2,Bax,Bak及肝细胞凋亡在丁型肝炎发病机制中的意义. 方法:采用TUNEL技术和免疫组化单、双标记染色,检测77例丁肝患者肝组织中HDAg,Bcl-2,Bax和Bak表达,以及肝细胞凋亡表达.同时以HDV阴性的67例乙型肝炎患者作对照. 结果:Bcl-2,Bax和Bak均以肝细胞质表达为主,HDAg 以肝细胞核表达为主.HDAg,Bax和Bak与肝细胞凋亡表达及分布有相关性(t=27.89,P<0.01,P<0.05 t=19.16, P<0.05 t=18.22),四成分在各型肝炎中的表达强度有显著陛羞别意义(P<0.05). 结论:HDAg,Bax,Bak和肝细胞凋亡表达强度及阳性细胞分布均与肝组织炎症和病理损害程度相关,HDV感染可诱导肝细胞表达Bax和Bak,增强肝细胞凋亡.
AIM: To study the expression of Bcl-2, Bax, Bak and hepatocytic apoptosis in pathogenesis of hepatitis D. METHODS: Expression of HDAg, Bcl-2, Bax, Bak and hepatocytic apoptosis in liver tissues of 77 patients with hepatitis D were studied by immunohistochemistry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) technique. Meanwhile, the relationship between expression of HDAg, Bcl-2/Bax and hepatocytic apoptosis were studied by double labeling and serial sections. RESULTS: Bcl-2 was mainly expressed in the cytoplasm of hepatocytes, Bax and Bak mainly in the cytoplasm of hepa-tocytes and partly in the nucleus of hepatocytes, and HDAg mainly in the nucleus of hepatocytes. Many HDAg and Bax or Bak positive cells were distributed among infiltrating lymophocytes at the periportal region especially at the advancing edges in the areas of piecemeal necrosis. Many apoptotic hepatocytes were found to locate near to positive cells of HDAg. There were positive correlations between degrees of HDAg expression, Bax or Bak expression, and hepatocytic apoptosis(t=27.89, P<0.01, P<0.05 t=19.16, P<0.05 t=18.22). CONCLUSION: The distribution and expression of Bax, Bak, HDAg and hepatocytic apoptosis are significantly correlated with the activity of imflammation and the severity of the liver damage.
出处
《世界华人消化杂志》
CAS
2004年第7期1578-1581,共4页
World Chinese Journal of Digestology
