摘要
目的 :观察中枢白细胞介素 1β (interleukin 1β ,IL 1β)在新奇应激 (noveltystress)诱发的大鼠运动行为 (mo torbehavior)反应中的作用。方法 :利用大鼠新奇环境应激箱诱发新奇应激 ;用大鼠脑立体定位技术与侧脑室埋管技术进行药物侧脑室微量注射 ,用行为遥感监测系统监测清醒大鼠运动行为反应 ,用行为学指标为描述大鼠静止行为 (immobility)的“静止行为平均百分数”(meanpercentimmobility ,MPI)。结果 :新奇应激可明显降低大鼠的MPI;非应激情况下 ,侧脑室预注射IL 1β特异性抗体不影响大鼠MPI;侧脑室预注射抗IL 1β抗体可拮抗新奇应激导致的大鼠MPI降低 ;新奇应激时 ,侧脑室预注射非特异性抗体分子IgG不影响大鼠MPI。结论 :中枢IL 1β参与调节新奇应激过程中大鼠运动行为反应的中枢调控。
Objective: To investigate the effect of central interleukin-1β (IL-1β) on motor behavioral responses in novelty stress rats. Methods: The novelty stress was elicited by novel environmental stimuli with novelty stress box. The intracerebrolventricular (ICV) cannula and microinjection were performed with rat brain stereotaxic system. Movement behaviors of rats were monitored by behavioral radio-telemetry system. As behavior index, mean percent immobility (MPI) was used to assess immobility of rats. Results: The decrease of MPI was remarkably elicited by novel environmental stimuli. In non-stressful condition, ICV anti-IL-1β antibody did not influence the MPI. The decrease of MPI induced by novel environmental stimuli was significantly blocked by ICV pretreatment with anti-IL-1β antibody. In novel stress, MPI of rats was not affected by ICV injection of non-specific IgG. Conclusion: Central interleukin-1β plays an important role in modulation of motor behavioral response to novelty stress.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2004年第3期244-247,共4页
Journal of Peking University:Health Sciences
基金
国家自然科学基金 ( 3 980 0 47
3 0 3 70 5 5 7)资助~~
