摘要
【目的】研究碱性成纤维细胞生长因子(bFGF)对大鼠骨髓基质干细胞(MSC)增殖的影响。【方法】用α-MEM冲洗骨髓腔,收集骨髓细胞悬液,接种在塑料培养瓶中,经体外扩增、纯化,观察其生长特性,用免疫组织化学SABC法检测波形蛋白和纤粘连蛋白的表达,并研究不同浓度bFGF对MSC生长曲线和克隆形成率的影响。【结果】原代培养时形成由基质干细胞组成的细胞集落,细胞集落14d时接近融合,传代后细胞体积变大,约5～7d传代1次;免疫组化显示MSC呈波形蛋白阳性,而纤粘连蛋白阴性。浓度10ng/mL和20ng/mLbFGF组的MSC的细胞数和克隆形成率比5ng/mLbFGF组和对照组(无bFGF)明显增加,具有高度显著性(P<0.01)。【结论】bFGF可明显促进MSC的增殖。
ObjectiveTo investigate the effect of base fibroblast growth factor (bFGF) on proliferation of bone mesenchymal stem cells (MSC)of SD rat. MethodsMSCs were isolated from young rat femur marrow flushed out with α-MEM using a needle and syringe, then planted in plastic culture flask. The proliferative characteristics of MSC were observed. Through expanded to 4 passages, vimentin and laminin were detected by immunohistochemistry. The effects of different concentration of bFGF on growth curve and clone-forming rate of MSC were studied. ResultsClones of MSC were formed in primary culture and contacted with one another at the 14th day. In passage cultured, the cells became somewhat bigger than that in primary culture and can be subcultured one generation in 5-7 days. The MSC showed positive expression of vimentin, but the expression of laminin was negative. The cell numbers and clone forming rates of MSC cultured with 10 ng/mL and 20 ng/mL bFGF groups were higher than those of 5 ng/mL bFGF group and control group (no bFGF). The differences were high significant (P< 0.01). ConclusionbFGF had significant effects on promoting the proliferation of MSCs.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2004年第6期508-511,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
国家重点基础研究(973)基金资助项目(G1999054301-2)
