摘要
针对两种主要的杜氏藻养殖种类D. salina 和 D .bardawil ,采用各种极性和非极性有机溶剂、不同的溶剂用量以及不同的超声波作用时间提取其中类胡萝卜素,得到的最佳提取条件是:以丙酮作提取溶剂,溶剂和培养液比为2:5,经超声波作用破壁5min。
The carotenoids in the two chiefly-raised algae, D. salina and D. bardawil, were extracted with various polar and apolar organic solvents, different use level of the solvents and different actuation durations of ultrasonication. The optimum extraction conditions of carotenoids from the two species were obtained: pure acetone as the extracting solvent, solvent, culture fluid(v/v)=2:5 and 5 min under ultrasonication.
基金
国家自然科学基金资助项目(20076020)