摘要
目的 探讨人胎儿和成人皮肤及其创面愈合过程中PDGF和EGF的表达及意义。方法 利用已建立的人胎儿无瘢痕愈合动物模型 ,获取相应标本 ,结合临床所取成人皮肤标本 ,采用免疫组化染色方法 ,观察PDGF、EGF的表达情况。结果 ①正常胎儿皮肤中未见明显的PDGF阳性染色 ;创伤后 12h、1d的胎儿表皮及真皮浅层可见PDGF的弱阳性表达 ;创伤后 3d、1周的胎儿皮肤中PDGF的表达呈阴性 ;正常成人皮肤可在成纤维细胞、巨噬细胞及毛细血管见到阳性表达 ;创伤后表达加强。②正常胎儿皮肤表皮全层和毛囊、皮脂腺及汗腺细胞可见EGF的阳性表达 ;创伤后的胎儿皮肤中EGF的表达未见到明显变化 ;正常成人皮肤可见表皮基底层有中度阳性表达 ,毛囊、汗腺细胞也可见到轻度表达 ,创伤后表达有所减弱。结论 生长因子在胎儿和成人皮肤创面愈合过程中的差异表达可能是胎儿无瘢痕愈合的重要原因之一。
Objective To explore the differences of PDGF and EGF expression in the wound healing between fatal and adult. Methods With the established animal model of fetal scarless healing and the adult samples, an immunohistochemical technique was used to evaluate the expression of PDGF and EGF in the normal adult skin, normal fetal skin, and the process of their wound healing. Results ① The expression of the PDGF was not found in the fetal skin, but a mild amount of the PDGF was shown in the epidermis and the upper dermal layer 12 hours and 1 day after the wounding process. In the normal adult skin, expression of PDGF was shown in the dermal fibroblasts, macrophagocytes and blood capillaries, and a strong expression was presented during its wound healing process. ②In the fetal skin, the expression of the EGF was seen in the epidermis, hair follicles, sebaceous glands and sweat glands, but there were no markedly changes during the wound healing. In the adult skin, a positive stain of the EGF was shown in the basal layer of the epidermis while the mild stain in hair follicles and sweat glands. The level of the expression became gradually decreasing with the time going in the wounded adult skin. Conclusion The different expression of growth factors between fetal and adult skin in wound healing may be one of the important reasons that the fetal wound could produce scarless healing.;
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2003年第3期199-202,共4页
Chinese Journal of Plastic Surgery
基金
国家自然科学基金资助 (项目编号 :30 0 0 0 174)
