摘要
目的 探讨吸氧对新生鼠视网膜的影响及其机制。 方法 高氧环境下用新生鼠视网膜的变化模拟早产儿视网膜病变 (retinopathy of prematurity,ROP)。将 118只新生鼠放入含氧 80 %的吸氧箱中连续饲养 7d,再置于空气中饲养 7d,以一直在空气中饲养的同龄鼠为对照组。分别检测 1、2、4、7、8、9、11、14 d龄鼠的动脉血氧分压及视网膜超氧化物歧化酶 (superoxide dismutase,SOD)、丙二醛 (malondi-aldehyde,MDA)的含量 ;血管灌注及密度计数检测 7、14 d龄鼠视网膜血管主干直径及周边血管覆盖率 ;HE染色检测 8、9、11、14 d龄鼠视网膜新生血管。 结果 实验组 1、2、4、7d龄鼠血氧分压明显高于对照组 (P<0 .0 1) ,8、9、11、14 d龄鼠血氧分压低于对照组 ,但差异无显著性的意义 (P>0 .0 5 ) ,实验组 1、2、4、7、8d龄鼠视网膜的 SOD含量低于对照组 (P<0 .0 1,P<0 .0 5 ) ,而 1、2、4、7、8d龄鼠 MDA高于对照组(P<0 .0 1,P<0 .0 5 ) ;实验组 7d龄鼠视网膜血管主干直径是对照组鼠的 75 % ,周边血管覆盖率是对照组的 2 2 % ,14 d龄鼠视网膜血管主干直径是对照组的 6 1% ,周边血管覆盖率为对照组的 73%。实验组中 ,16 .7%的鼠视网膜发现新生血管 ,而对照组未发现。
Objective To explore the effect of oxygen inhalation on the retinae of newborn rats and its mechanism. Methods We mimicked the retinopathy of prematurity(ROP) by putting the newborn rats in high concentrated oxygen. One-day-old rats were put into the oxygen box with the oxygen concentration of 80% for continuous 7 days; then in air condition for 7 days. The arterial blood oxygen pressure, retinal superoxide dismutase (SOD), and malondialdehyde (MDA) of the rats (1,2,4,7,8,9,11,14 days old) were examined. The diameter of retinal vessels′ main branch and the coverage rate of peripheral vessels were measured in 7- and 14-day-old rats by ink perfusion. The retinal neovascularization of rats (8,9,11,14 days old) were observed by HE staining. The rats of the same age fed in air condition were in the control group. Results The differential pressures of blood oxygen of rats (1,2,4,7 days old) in study group were significantly higher than those in the control group (P<0.01), while the differential pressures of blood oxygen of rats (8,9,11,14 days old) in study group were lower than those in the control group (P>0.05). The contents of SOD of the retinae in the rats (1,2,4,7,8 days old) were significantly lower than those in the control group(P<0.01,P<0.05), while the contents of MDA were significantly higher than those in the control group (P<0.01,P<0.05). The diameter of retinal vessels′ main branch in 7-day rats was 75% of the control group, and the coverage rate of peripheral vessels was 22% of the control group; and was 61% and 73% respectively in 14-day-old rats. The neovascularization could be seen in 16 7% of the rats in the study group and nought in the control group. Conclusion The damage of free radical of the retina in high concentrated oxygen and hypoxia situation after oxygen supply may be one of the most important mechanism of ROP.
出处
《中华眼底病杂志》
CAS
CSCD
2003年第5期306-309,共4页
Chinese Journal of Ocular Fundus Diseases
