摘要
用化学[(氨甲蝶呤(MTX)、胸腺嘧啶核苷(TdR)]及物理(冷冻休克)法,对培养的新生乳兔肺成纤维细胞进行同步化处理,常规收获细胞,制备染色体标本,行胰酶G显带法,制得兔染色体高分辨G带核型。分析了各条染色体带型特征,绘制出583条带的模式图。并对兔染色体高分辨带型、实验手段等问题进行了探讨。将有助于基因定位、染色体、进化理论和肿瘤染色体特性等工作的研究。
Since rabbit (Oryctolagus cuniculus) possess many fine biological characteristics, they have been used in the studies of tumour, gene mapping and evolution. All of these need us to know the high-resolution G-banding pattern of rabbit chromosomes. Primary lung fibroblasts of new born rabbits were cultivated in RPMI 1640 medium supplemented with 20% new born bovine serum. In order to get the synchronized cells, chemical(MTX, TdR) method and physical (Cold Shock) method were used. By using trypsin-Giemsa banding-technique, we obtained fine high-resolution G-banding karyotypes of rabbit. The bands of rabbit chromosomes were analyzed and described. An idiogram which contains 583 bands of high-resolution G-banding chromosomes of rabbit has been made. The feature of rabbit choromosome bands and the method uesd to get the high resolution banding chromosomes were briefly discussed.
出处
《解剖学报》
CAS
CSCD
北大核心
1993年第1期63-67,共5页
Acta Anatomica Sinica
关键词
染色体
高分辨带型
兔
Chromosome
High resolution G banding
Rabbit
