摘要
本实验应用细胞培养、扫描电镜、透射电镜及放射免疫等技术,以形态和功能相结合的方法,研究了三氯化钐(SmCl_3)对腺垂体细胞形态、超微结构及功能的影响.SmCl_3作用后,放免测定实验组细胞上清液中GH、LTH浓度较对照组明显增高,而TSH、FSH及LH浓度较对照组明显降低.电镜观察:实验组嗜酸性细胞质内的粗面内质网扩张、高尔基复合体发达,分泌颗粒增多且移向细胞的周边,细胞呈分泌及合成功能旺盛相;而实验组嗜碱性细胞数量减少,胞质中的分泌颗粒减少,细胞呈分泌及合成功能抑制状态,说明SmCl_3对腺垂体嗜酸性细胞的合成及分泌激素的功能有促进作用,对腺垂体嗜碱性细胞的合成及分泌激素的功能有抑制作用.
The effects of samarium chloride (SmCl_3) on rat adenohypophyseal cells in vitro were studied with radioimmunoassay (RIA) and electron microscopy.The adenohypophyseal cells were exposed to 0.01 mmol/L and 1 mmol/L,of SmCl_3 respectively for 24 hours.Compared with the control,the concentrations of growth hormone (GH) and lactotropic hormone (LTH) in the supernatant were increased significantly,while the values of thyroidstimulating hormone (TSH),follicle stimulating hormone (FSH) and luteinizing hormone (LH) were decreased markedly.This study demonstrates that SmCl_3 exerts a stimulating effect on the synthesis and secretion of GH and LTH,and an inhibiting effect on the synthesis and secretion of TSH,FSH and LH.
出处
《解剖学杂志》
CAS
CSCD
北大核心
1993年第1期31-34,共4页
Chinese Journal of Anatomy
关键词
腺垂体
细胞
三氯化钐
电子显微镜
adenohypophyseal cells
cell culture
radioimmunoassay
