期刊文献+

Lymphatic metastasis and nm23H_1 genetic instability in Chinese colon cancer patients 被引量:7

Lymphatic metastasis and nm23H_1 genetic instability in Chinese colon cancer patients
下载PDF
导出
摘要 AIM: To investigate the pathogenic mechanism of colon cancer at the molecular level and to elucidate the relationship between intercellular adhesion molecule-1 (ICAM-1) and nm23H1 genes and Chinese patients with colon cancer.METHODS: DNA was extracted from paraffin-embedded materials. Polymerase chain reaction-single s^and conformation polymorphism (PCR-SSCP) was used to analyze MSI and LOH. Expression of ICAM-1 was detected by Envision immuno-histochemistry. Experimental results were analyzed with Leica-Qwin computer imaging techniques and SPSS software of statistics.RESULTS: ICAM-1 expression of lymphatic endothelium was negative in normal colon and posi0ve in colon cancer respectively. The number of lymphatics positive for ICAM-1 was gradually increased with degree of cancer invasion (P<0.01). In the group with metastasis of colon cancer, bhe number of lymphatics positive for ICAM-1 in lymph nodes was more than that in the group with no metastasis (P<0.01). The frequency of MSI, LOH and nm23H1 protein was 26.67%, 20.00% and 53.33% in colon cancer, respectively. In TNM staging, MSI (43.75%) and nm23H1 protein (81.25%) in stages I+II weredetected more easily than the corresponding indexes (MSI:7.14%, P<0.05 and nm23H,: 21.43%, P<0.01) in stages III+IV. By comparison, the frequency of LOH (35.71%) in stages III+IV was more than that of LOH (6.25%, P<0.05) in stages I+II. LOH exhibited a rising trend along with the Duke's staging, nm23H1 protein in the group of tubular adenocarcinoma (60.00%) was higher expressed than that in the group of mucoid adenocarcinoma (20.00%) (P<0.01),and exhibited a rising trend with the differentiation degrees of tubular adenocarcinoma, nm23H, protein in MSI positive group was higher expressed (75%) than that in MSI negative group (45.45%, P<0.05).CONCLUSION: The expression of ICAM-1 in lymphatic vessels is beneficial to the judgement of the invasion and metastasis ability of colon cancer and the anti-tumor immunity function,and shows an important clinical significance in predicting lymphatic metastasis of colon cancer. MSI and LOH may separately control the development of sporadic colon cancer with different pathways. LOH mostly arises in the late period of sporadic colon cancer and endows a high aggressive and poor prognostic phenotype. By compassion, MSI may be an early period molecule marker for sporadic colon cancer, enhanced expression of nm23H1 protein can effectively inhibit colon cancer metastasis and improve prognosis of sporadic colon cancer patients. AIM:To investigate the pathogenic mechanism of colon cancer at the molecular level and to elucidate the relationship between intercellular adhesion molecule-1(ICAM-1)and nm23H_1 genes and Chinese patients with colon cancer. METHODS:DNA was extracted from paraffin-embedded materials.Polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP)was used to analyze MSI and LOH.Expression of ICAM-1 was detected by Envision immuno- histochemistry.Experimental results were analyzed with Leica-Qwin computer imaging techniques and SPSS software of statistics. RESULTS:ICAM-1 expression of lymphatic endothelium was negative in normal colon and positive in colon cancer respectively. The number of lymphatics positive for ICAM-1 was gradually increased with degree of cancer invasion(P<0.01).In the group with metastasis of colon cancer,the number of lymphatics positive for ICAM-1 in lymph nodes was more than that in the group with no metastasis(P<0.01).The frequency of MSI,LOH and nm23H_1 protein was 26.67%,20.00% and 53.33% in colon cancer,respectively.In TNM staging,MSI (43.75%) and nm23H_1 protein(81.25%)in stages Ⅰ+Ⅱ were detected more easily than the corresponding indexes(MSI: 7.14%,P<0.05 and nm23H_1:21.43%,P<0.01)in stages Ⅲ+Ⅳ.By comparison,the frequency of LOH(35.71%)in stages Ⅲ+Ⅳ was more than that of LOH(6.25%,P<0.05) in stages Ⅰ+Ⅱ.LOH exhibited a rising trend along with the Duke's staging,nm23H_1 protein in the group of tubular adenocarcinoma(60.00%)was higher expressed than that in the group of mucoid adenocarcinoma(20.00%)(P<0.01), and exhibited a rising trend with the differentiation degrees of tubular adenocarcinoma,nm23H_1 protein in MSI positive group was higher expressed(75%)than that in MSI negative group(45.45%,P<0.05). CONCLUSION:The expression of ICAM-1 in lymphatic vessels is beneficial to the judgement of the invasion and metastasis ability of colon cancer and the anti-tumor immunity function, and shows an important clinical significance in predicting lymphatic metastasis of colon cancer.MSI and LOH may separately control the development of sporadic colon cancer with different pathways.LOH mostly arises in the late period of sporadic colon cancer and endows a high aggressive and poor prognostic phenotype.By compassion,MSI may be an early period molecule marker for sporadic colon cancer, enhanced expression of nm23H_1 protein can effectively inhibit colon cancer metastasis and improve prognosis of sporadic colon cancer patients.
出处 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第19期2800-2804,共5页 世界胃肠病学杂志(英文版)
  • 相关文献

参考文献32

  • 1Bodey B, Bodey B Jr, Siegel SE, Kaiser HE. Failure of cancer vaccines: the significant limitations of this approach to immunotherapy. Anticancer Res 2000; 20:2665-2676.
  • 2Dudda JC, Simon JC, Martin S. Dendritic cell immunization route determines CD8~ T cell trafficking to inflamed skin: role for tissue microenvironment and dendritic cells in establishment of T cell-homing subsets. J Immunol 2004; 172- 857-863.
  • 3Launay D, Peyrat JP, Verdiere A, Hornez L, Hatron PY,Hachulla E, Devulder B, Hebbar M. Multiplex reverse transcription polymerase chain reaction assessment of sialyltransferase expression in peripheral blood mononuclear cells in systemic sclerosis. J Rheumatol 2004; 31:88-95.
  • 4Perdikogianni CH, Dimitriou H, Stiakaki E, Markaki EA,Kalmanti M. Adhesion molecules, endogenous granulocyte colony-stimulating factor levels and replating capacity of progenitors in autoimmune neutropenia of childhood. Acta Paedfatr 2003; 92:1277-1283.
  • 5Kwei S, Stavrakis G, Takahas M, Taylor G, Folkman MJ,Gimbrone MA Jr, Garcia-Cardena G. Early adaptive responses of the vascular wall during venous arterialization in mice. Am J Pathol 2004; 164:81-89.
  • 6Benkoel L, Dodero F, Hardwigsen J, Benoliel AM, Bongrand P,Botta-Fridlund D, Le Treut YP, Chamlian A, Lombardo D.Expression of intercellular adhesion molecule-1 (ICAM- 1) during ischemia-reperfusion in human liver tissue allograft:image analysis by confocal laser scanning microscopy. Dig Dis Sci 2003; 48:2167-2172.
  • 7Nyska A, Moomaw CR, Ezov N, Shabat S, Levin-Harrus T,Nyska M, Redlich M, Mittelman M, Yedgar S, Foley JF. Ocular expression of vascular cell adhesion molecule (VCAM-1) in 2butoxyethanol-induced hemolysis and thrombosis in female rats. Exp Toxicol Pathol 2003; 55:231-236.
  • 8Somersalo K, Anikeeva N, Sims TN, Thomas VK, Strong RK,Spies T, Lebedeva T, Sykulev Y, Dustin ML. Cytotoxic T lymphocytes form an antigen-independent ring junction. J Clin Invest 2004; 113:49-57.
  • 9Chattopadhyay R, Taneja T, Chakrabarti K, Pillai CR, Chimis CE. Molecular analysis of the cytoadherence phenotype of a Plasmodium falciparum field isolate that binds intercellular adhesion molecule-1. Mol Biochem Parasitol 2004; 133:255-265.
  • 10Einstein O, Karussis D, Grigoriadis N, Mizrachi-Kol R,Reinhartz E, Abramsky O, Ben-Hut T. Intraventricular transplantation of neural precursor cell spheres attenuates acute experimental allergic encephalomyelitis. Mol Cell Neurosci 2003;24:1074-1082.

同被引文献30

引证文献7

二级引证文献21

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部