摘要
AIM: To investigate the protective effect of lansoprazoleon ischemia and reperfusion (I/R)-induced rat intestinalmucosal injury in vivo.METHODS: Intestinal damage was induced by clampingboth the superior mesenteric artery and the celiac trunkfor 30 rain followed by reperfusion in male Sprague-Dawleyrats. lansoprazole was given to rats intraperitoneally 1 hbefore vascular clamping.RESULTS: Both the intraluminal hemoglobin and proteinlevels, as indices of mucosal damage, significantlyincreased in I/R-groups comparion with those of sham-operation groups. These increases in intraluminal hemoglobinand protein levels were significantly inhibited by the treatmentwith lansoprazole at a dose of 1 mg/kg. Small intestineexposed to I/R resulted in mucosal inflammation that wascharacterized by significant increases in thiobarbituric acid-reactive substances (TBARS), tissue-associatedmyeloperoxidase activity (MPO), and mucosal content of ratcytokine-induced neutrophil chemoattractant-1 (CINC-1).These increases in TBARS, MPO activities and CINC-1 contentin the intestinal mucosa after I/R were all inhibited bypretreatment with lansoprazole at a dose of 1 mg/kg.Furthermore, the CINC-1 mRNA expression was increasedduring intestinal I/R, and this increase in mRNA expressionwas inhibited by treatment with lansoprazole.CONCLUSION: Lansoprazole inhibits lipid peroxidation andreduces development of intestinal mucosal inflammationinduced by I/R in rats, suggesting that lansoprazole mayhave a therapeutic potential for I/R injury.
AIM:To investigate the protective effect of lansoprazole on ischemia and reperfusion(I/R)-induced rat intestinal mucosal injury in vivo. METHODS:Intestinal damage was induced by clamping both the superior mesenteric artery and the celiac trunk for 30 rain followed by reperfusion in male Sprague-Dawley rats.Lansoprazole was given to rats intraperitoneally 1 h before vascular clamping. RESULTS:Both the intraluminal hemoglobin and protein levels,as indices of mucosal damage,significantly increased in I/R-groups comparion with those of sham- operation groups.These increases in intraluminal hemoglobin and protein levels were significantly inhibited by the treatment with lansoprazole at a dose of 1 mg/kg.Small intestine exposed to I/R resulted in mucosal inflammation that was characterized by significant increases in thiobarbituric acid- reactive substances(TBARS),tissue-associated myeloperoxidase activity(MPO),and mucosal content of rat cytokine-induced neutrophil chemoattractant-1(CINC-1). These increases in TBARS,MPO activities and CINC-1 content in the intestinal mucosa after I/R were all inhibited by pretreatment with lansoprazole at a dose of 1 mg/kg. Furthermore,the CINC-1 mRNA expression was increased during intestinal I/R,and this increase in mRNA expression was inhibited by treatment with lansoprazole. CONCLUSION:Lansoprazole inhibits lipid peroxidation and reduces development of intestinal mucosal inflammation induced by I/R in rats,suggesting that lansoprazole may have a therapeutic potential for I/R injury.
