摘要
研究了补糖对荧光假单胞菌(Pseudomonas fluorescens)AR4生产2-酮基-D-葡萄糖酸的影响,并在50kL发酵罐上进行了补料分批培养工业应用性试验。研究结果表明,采用补料分批培养方法,能有效提高发酵液中的产物浓度;开始补糖时发酵液中的残糖浓度以3%～6%为宜;补料分批培养方法及AR4菌株可用于2-酮基-D-葡萄糖酸的工业化规模生产中。
The effects of feeding glucose on the production of 2-keto-D-gluconic acid by Pseudomonas fluorescens AR4 werestudied, and the application of fed-batch culture in the industrial production was also investigated in a 50kL fermentor. The resultsshowed that the concentration of the product could be increased effectively in fed-batch culture, the suitable concentration ofresidual glucose in the fermentation broth was 3%～6% while beginning to feed glucose, and the culture method and the strainAR4 could be applied in the industrial fermentation process.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2004年第11期115-117,共3页
Food Science
