摘要
目的 :探讨在非清髓性预处理条件下 ,纯化的供鼠 NK细胞对 MHC半相合小鼠骨髓移植 (BMT)造血重建的影响。方法 :以 CB6 F1 H- 2 d/b (BAL B/c H- 2 d× C5 7BL/6 H- 2 b)小鼠为受者 ,C5 7BL/6 H- 2 b小鼠为供者。 6 0只受者小鼠根据不同照射剂量及不同 NK细胞输注量随机分为 10组 ,每组 6只 ,经 9.0、 7.0、 6 .0 Gy 3个剂量 6 0 Co照射。经 9.0 Gy照射后分为单纯照射组 ( )、半相合 BMT组 ( )。经 7.0 Gy照射后分为单纯照射组 ( )、半相合 BMT组 ( )、 NK细胞移植 1组 ( )和 NK细胞移植 2组 ( )。经 6 .0 Gy照射后分为单纯照射组( )、半相合 BMT组 ( )、 NK细胞移植 1组 ( )和 NK细胞移植 2组 ( )。在照射后 4 h移植供鼠骨髓细胞 ,其中 NK细胞移植 1组 ( 、 )每只受鼠移植前输注纯化供鼠 NK细胞 1× 10 6 ,NK细胞移植 2组 ( 、 )每只受鼠移植前输注供鼠 NK细胞 5× 10 5。以血常规、体重、生存期等变化为指标 ,观察 6 0 d。结果 :1生存期 : 组为 (5 .83± 0 .98) d,其他组小鼠生存期均大于 6 0 d。 2移植后第 10天时白细胞及血小板计数 : 、 组 WBC和 PL T分别为 (1.0 5± 0 .30 )× 10 9· L- 1、 (5 3.5 0± 12 .11)× 10 9· L- 1及 (0 .95± 0 .2 6 )× 10 9· L- 1、(4 7.17± 11.82 )× 10 9·
Objective To study the relationship between donor-derived NK cells and hematopoietic reconstitution after nonmyeloablative MHC haplotype-mismatched BMT in mice. Methods The murine model of MHC haplotype-mismatched BMT was established by using (BALB/c H-2d ×C57BL/6 H-2b ) CB6F 1 H-2d/b mouse as the recipient, and C57BL/6 H-2b mouse as the donor. 60 recipient mice were divided into 10 groups after irradiation (TBI 60 Co 9.0,7.0, and 6.0 Gy):9.0 Gy-irradiation groups including simple-irradiation group (Ⅰ), MHC haplotype-mismatched BMT group(Ⅱ); 7.0 Gy-irradiation groups including simple-irradiation group (Ⅲ), MHC haplotype-mismatched BMT group (Ⅳ), the first of NK cells-transplantation group (Ⅴ), the second of NK cells-transplantation group (Ⅵ), 6.0 Gy-irradiation groups including simple-irradiation group (Ⅶ), MHC haplotype-mismatched BMT group (Ⅷ), the first of NK cells-transplantation group (Ⅸ), the second of NK cells-transplantation group (Ⅹ). The recipients were infused into the bone marrow cells of donors when they were irradiated after 4 hours. The recipients, the first of NK cells-transplantation groups, were infused into NK cells of 1×10 6/one; The recipients, the second of NK cells-transplantation groups, were infused into NK cells of 5×10 5/one. The effects were assessed by the count of WBC and PLT, survival time, body weight in the recipients. Results ①Life span: survival time was (5.83±0.98) days in group Ⅰ, beyond 60 days in other groups. ②Leukocyte and platelet counts after 10 days: leukocyte and platelet counts were (1.05±0.30)×10 9· L -1 , (53.50±12.11) ×10 9·L -1 and (0.95±0.26)×10 9·L -1 ,(47.17±11.82)×10 9·L -1 in group Ⅴ and group Ⅵ, which were much higher than those in group Ⅲ and group Ⅳ (P<0.01); In the cases of group Ⅸ and group Ⅹ, leukocyte and platelet counts were (0.98±0.34)×10 9·L -1 ,(50.67±12.58)×10 9·L -1 ;(0.88±0.29)× 10 9·L -1 , (45.33±14.36)×10 9·L -1 , which were much higher than those in group Ⅶ and group Ⅷ (P<0.05). Conclusion Donor-derived NK cells can promote hematopoietic reconstitution after nonmyeloablative MHC haplotype-mismatched BMT in mice.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2004年第6期908-911,共4页
Journal of Jilin University：Medicine Edition
基金
全军医药卫生科研基金十五重点项目资助课题 (0 1Z0 5 0 )
关键词
杀伤细胞天然
骨髓移植
非清髓性
造血重建
kill cells, natural
bone marrow transplantation
nonmyeloablative
hematopoietic reconstitution
