摘要
空间微重力环境会影响生命活动,本文利用回转器来模拟微重力(失重)的生物学效应.回转处理可以引起胡萝卜愈伤组织细胞中内源活性赤霉素(GAs)水平上升,总淀粉酶活力提高(其中主要是α-淀粉酶活力增加),淀粉粒降解.且GAs和α-淀粉酶活力的升高趋势一致.GAs合成性抑制剂,ancymidol能显著抑制回转处理中α-淀粉酶活力的增加.由此推测:回转处理中GAs的升高诱导了α-淀粉酶基因表达增加,后者促使淀粉粒降解和能量物质动员,以响应模拟失重的刺激.
Microgravity environment in space affects organism. The biological effects of microgravity have been used a clinostat to simulate. The clinostat treatment induced the increase of gibberellins (GAs) and the activity of total amylase (especially the increment of a-amylase activity), as well as the degradation of starch grains in carrot callus cells. The increment trend of GAs and α-amylase activity was con-sistent. Further more, ancymidol, the inhibitor of the biosynthesis of GAs could remarkably repress the increment of α-amylase activity induced by the clinostat treatment. Therefore, we deduce that clinostat treatment can stimulate the raise of GAs, which induces the increment of the expression of α-amylase genes, then trig-gers the degradation of starch grains. As a result, plant can produce more active energy to respond to clinostat stimulus.
出处
《空间科学学报》
CAS
CSCD
北大核心
2004年第5期394-400,共7页
Chinese Journal of Space Science
基金
中国科学院知识创新工程重要方向项目(KSCX2-SW-322)
国家自然科学基金项目(39770199)共同资助
