摘要
以东方百合 索邦 L ilium orential Sorbonne 的花梗、花托、花瓣和花丝为外植体 ,进行了离体培养与快速繁殖研究 .结果表明 ,4种花器官均可直接诱导产生不定芽 ,诱导不定芽的适宜培养基为 N6 +BA1.0 m g· L- 1+KT 1.0 mg· L- 1 +NAA 0 .2 m g· L- 1 ;芽增殖的适宜培养基为 MS+BA 1.0 mg· L- 1 +NAA 0 .1m g· L- 1 ;生根与小鳞茎增殖的适宜培养基为 1/ 2 MS+IBA0 .1～ 0 .2 mg· L- 1 .适宜的移栽基质为珍珠岩与营养土 3∶ 1
Floral organ culture and rapid propagation of Lilium orential “Sorbonne” were carried out using pedicle, receptacle, petal, and filament as explants. The results showed that adventitious buds could be induced directly form four kinds of floral organs and the appropriate medium was N_6 + BA 1.0 mg·L^(-1) + KT 1.0 mg·L^(-1) + NAA 0.2 mg·L^(-1). The optimum proliferation medium for adventitious buds was MS + BA 1.0 mg·L^(-1) + NAA 0.1 mg·L^(-1). The appropriate medium for rooting and proliferation of bulblet was 1/2 MS + IBA 0.1～0.2 mg·L^(-1). The suitable transplantation matrix for bulblets was 3/4 pearlite + 1/4 nutrient soil.
出处
《西北植物学报》
CAS
CSCD
2004年第13期2350-2354,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金项目 3 0 3 710 13
关键词
东方百合“索邦”
花器官培养
快速繁殖
Lilium orential “Sorbonne”
floral organ culture
rapid propagation
