摘要
目的研究山茶花总黄酮(TFC)对缺血性脑损伤的保护作用。方法采用断头法、闷罐法,观察TFC对脑缺血小鼠耐缺氧能力的影响;采用跳台法观察TFC对缺血后学习记忆功能的影响;并测定脑组织中丙二醛(MDA)、一氧化氮(NO)的含量和乳酸脱氢酶(LDH)活性。采用结扎双侧颈总动脉(CCA)制作大鼠急性不完全性脑缺血模型,观察TFC对脑含水量、血管通透性及组织病理形态的影响。结果TFC可延长小鼠断头后喘息持续时间和在缺氧条件下的存活时间,改善缺血后引起的学习记忆障碍,降低缺血后小鼠脑组织中MDA和NO含量,抑制LDH活性的下降;并能降低大鼠CCA结扎后脑含水量和血管通透性,改善脑组织病理形态学改变。结论TFC对缺血性脑损伤有显著的保护作用,其机制可能与抗自由基、抑制NO的生成有关。
Objective To observe the protective effect of total flavone of Camellia (TFC)against cerebral ischemic injury.Methods Decapitation method and close hypoxia method were used to observe the effect of TFC on anoxic tolerance of mice and step down test was used to observe the effect of TFC on learning and memory; after ischemia, the content of malondialdehyde (MDA)and nitric oxide (NO)and the activity of lactate dehydrogenase (LDH)were detected. Rat models with acute incompletely cerebral ischemia were established by means of ligating right common carotid arteries and effect of TFC on cerebral water volume, permeability of cerebral vessels and cerebral histopathological changes were also observed.Results TFC prolonged the grasping time after decapitation and the survival time after anoxia in mice, and improve the learning and memory during the step down test. TFC decreased MDA and NO contents, counteracted the de creases of LDH activities in the mice cerebral cortex, reduced the water volume and permeability of cerebral vessels in ischemic rats and improve the cerebral hitstopathological changes. Conclusion TFC has protective effects against cerebral ischemic injury and the mechanism may be related to the inhibition of free radicals and NO production.
出处
《中药新药与临床药理》
CAS
CSCD
2004年第6期376-379,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
安徽自然科学基金资助项目(编号99044433)
