摘要
目的 :研究血管内皮生长因子 (VEGF)、诱导型一氧化氮合酶 (iNOS)和内皮型一氧化氮合酶 (eNOS)在口腔鳞癌中表达的相关性 ;探讨三者与口腔鳞癌血管生成和临床病理特征的关系 ;研究一氧化氮 (NO)和VEGF的相互作用及NO在VEGF促肿瘤生长中的作用机制。方法 :应用免疫组织化学方法检测 64例口腔鳞癌手术切除标本VEGF、iNOS和eNOS的表达 ,Ⅷ因子相关抗原 (FⅧRAg)血管内皮细胞特异性染色 ,计数肿瘤微血管密度 (MVD )。结果 :( 1) 3 9例口腔鳞癌组织表达VEGF ,42例表达iNOS ,45例表达eNOS ;( 2 )VEGF与iNOS的表达正相关 ,与eNOS的表达无相关 ;( 3 )VEGF、iNOS的表达与口腔鳞癌MVD呈正相关 ,eNOS的表达与口腔鳞癌MVD的差异无显著性意义 ;( 4 )VEGF的表达与口腔鳞癌淋巴结转移和肿瘤分化程度正相关 ;与临床分期无关。iNOS表达与口腔鳞癌的分化程度及临床分期和有无淋巴结转移正相关 ;eNOS表达与口腔鳞癌临床分期和分化程度及有无淋巴结转移均无关。结论 :( 1)iNOS对VEGF的生成和发挥作用的过程有重要影响 ;( 2 )MVD随着VEGF和iNOS表达的增强而增加 。
Objective: To study the relation among inducible nitric oxide synthase(iNOS), endothelial nitric oxide synthase(eNOS) and vascular endothelial growth factor (VEGF)in oral squamous cell carcinoma(OSCC) and their relation to clinical pathology and angiogenesis.Methods:OSCC tissue specimens were surgically obtained from 64 patients,the expression of iNOS,eNOS and VEGF in the samples were studied by immunohistochemistry technique. Microvessel density(MVD) was measured by counting the endothelial cells stained with anti-FⅧRAg antibody. Results: ①Positive VEGF and iNOS and eNOS immunostaining was detected in 39(60.94%),42(65.63%)and 45( 70.31%)of the cases respectively.②Expression of VEGF was significantly related to that of iNOS(P<0.01), but not eNOS(P>05).③MVD in OSCC tissue was positively correlated with the expression of VEGF and iNOS(P< 0.01),but not eNOS(P>05).④VEGF expression was positively correlated to the pathological grade of OSCC(P<0.05),and to the lymph metastasis(P<0.01),iNOS was positively correlated with pathological grade of OSCC(P<0.05),lymph metastasis(P<0.01) and TNM phase(P<0.05).eNOS showed no relation to above clinico-pathological features(P>0.05).Conclusion:iNOS,rather than eNOS, plays a positive role in OSCC development.iNOS and VEGF have a positive correlation in angiogenesis of OSCC.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2004年第6期720-723,共4页
Journal of Practical Stomatology
关键词
口腔
鳞状细胞癌
血管内皮生长因子
一氧化氮合酶
血管生成
Mouth
Squamous cell carcinoma
Vascular endothelial growth factor
Nitric oxide synthase
Angiogenesis
