摘要
目的 研究c erbB 2在肾细胞癌中的表达及其与临床分期、病理分型、病理分级和预后的关系。方法 应用免疫组化S P法分别用鼠抗人c erbB 2胞内段单克隆抗体 (CB11)和鼠抗人c erbB 2胞外段单克隆抗体 (9G6 10 )检测 77例肾细胞癌及相应癌旁肾组织c erbB 2蛋白表达。应用RT PCR方法检测 10例新鲜肾癌组织及癌旁正常组织c erbB 2mRNA的表达。透明细胞癌、颗粒细胞癌及乳头状肾细胞癌c erbB 2的表达分别为 6 8 2 %、93 1%及 75 0 %。结果 77例肾癌组织CB11和9G6 10的阳性率分别为 6 1% (4 7/77)和 4 5 5 % (35 /77) ;二者联合检测总阳性率为 77 9% (6 0 /77)。透明细胞癌、颗粒细胞癌及乳头状肾细胞癌c erbB 2的表达分别为 6 8 9%、92 9%及 75 %。RT PCR检测新鲜肾癌组织c erbB 2mRNA的阳性率为10 0 % (10 /10 )。结论 肾癌中c erbB 2蛋白及c erbB 2mRNA均过表达。不同的肾癌病理类型c erbB 2蛋白表达不同 ,颗粒细胞癌表达最高。c erbB 2在肾癌的不同临床分期均过表达 ,说明可能在病变的早期c erbB 2已经发生改变。c erbB
Purpose To study the expression of c erbB 2 in human renal cell carcinoma (RCC) and its relationship with clinical stages, histological classification, histopathologic grade and prognosis of RCC Methods Immunohistochemical assays were employed to analyze c erbB 2 expression in primary renal specimens Two types of mouse anti human c erbB 2 specific monoclonal antibodies were used respectively: CB11 was detected against the intracellular epitope and 9G6.10 was detected against the extracellular domain of c erbB 2 RT PCR were performed in fresh tumor tissue and corresponding normal renal tissue in 10 cases of RCC Results The positive rate of CB11 and 9G6 10 in RCC tissue was 61% (47/77) and 45 5% (35/77) respectively, and the overall positive rate was 77 9% (60/77) c erbB 2 positivities for both antibodies were demonstrated in 68 2% of clear cell carcinoma, 93 1% of granular cell carcinoma and 75% of papillary cell carcinoma The positive rate of c erbB 2 mRNA detected by RT PCR method in fresh tissue of all 10 cases of RCC was 100%. Conclusions The overexpression of c erbB 2 is detected in RCC tissue A distinctive c erbB 2 expression pattern is found in different subtypes of RCC with the highest frequency in granular cell carcinomas c erbB 2 is naturely processed by RCC c erbB 2 expression is neither associated with tumor grades nor with clinical stages
出处
《临床与实验病理学杂志》
CAS
CSCD
2003年第2期177-180,共4页
Chinese Journal of Clinical and Experimental Pathology
