摘要
目的 研究室温保存下白细胞凋亡的时相变化及Bcl 2和Bax在其中的作用。方法 采用Annexin V FITC -PI双标染色 ,结合流式细胞术 (FCM)检测细胞凋亡 ;以Westernblot检测Bcl 2和Bax蛋白表达变化。结果 1～ 2 4h凋亡细胞百分数逐渐增加 ,在 2 4h时达到峰值 ;72h时凋亡细胞反而降低。随着保存时间的延长 ,坏死细胞呈由低到高的变化 ,而活细胞则越来越少。Westernblot结果显示 ,1～ 2 4hBax表达逐渐增加 ,条带越来越清晰 ;72h时表达反而降低。 1～ 72h内 ,Bcl 2表达逐渐降低。结论 室温保存下 ,白细胞逐渐凋亡坏死 ,活细胞数量逐渐减少 ;Bcl 2和Bax在调节白细胞凋亡的机制中起着重要的作用。临床应用以输用保存 3～
Objective To study the roles of Bcl 2 and Bax in the time dependent changes of leukocyte apoptosis under room temperature conservancy. Methods Flow cytometry and Annexin V FITC PI double label staining were used to detect apoptosis, and Western blot was employed to determine the protein expressions of Bcl 2 and Bax. Results The percentage of apoptotic cells increased gradually since the 1st h and reached a peak at 24 h, but decreased at 72 h. The number of apoptotic cells increased in a time dependent manner. Western blot analysis demonstrated that the expression of Bax increased gradually during the period of 1～24 h and decreased at 72 h, but the expression of Bcl 2 decreased during 1～72 h. Conclusion The number of living cells conserved at room temperature decreased in a time dependent manner. Bcl 2 and Bax may play an important role in the regulatory mechanism in leukocyte apoptosis. Leukocytes conserved for 1～3 h may be suitable for clinical application.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2003年第13期1185-1187,共3页
Journal of Third Military Medical University
