摘要
应用银染技术对114例结肠癌,95例结肠腺瘤和10例正常结肠进行核仁形成区嗜银蛋白(Ag-NORs)数量、形态、大小和分布的定量研究。结肠癌的Ag-NORs均数/核(7.04 ±2.20)明显高于结肠腺瘤(3.43±0.88,P=0.0000)和正常结肠(1.25±0.09,P=0.0000)。结肠癌的异形Ag-NORs均数/100个核(61.39土22.38)明显高于结肠腺瘤(20.74土26.61,P=0.0000)和正常结肠(0,P=0.0000)。结肠癌的小型(<lum)和大型(>2um)Ag-NORs均数/100个核(483.76±242·89和)78.08±23.06)明显高于结肠腺瘤(112.00±47·23和19·49±17.47 P值均=0.0000)和正常结肠(0和0.76土1.16,P值均=0.0000)。结肠癌Ag-NORs颗粒的聚合、分散、核仁内和混合型分布(23.64％、53.20％、9.30％和13.86％)明显不同于结肠腺瘤(77.72％、16.88％、5.46％和0.04％,P值均<0.05)和正常结肠(100％、0、0和0、P值均<0.05)。本研究结果提示Ag-NORs4项定量指标有助于结肠良恶性病变的鉴别诊断。
In the present study, silver-stained nucleolar organizer regions (Ag-NORs) were studied with quantitative methods in paraffin sections of 114 colorectal carcinomas, 95 colorectal adenomas and 10 normal colorectal mucosa. The number, shape, size and distribution of Ag-NORs were counted and analysed. The purpose of this study was to probe into the diagnostic significance of qualitative evaluation of Ag-NORs in malignant and benign tumors of the colon. The results showed: The number of Ag-NORs in colorectal carcinomas (7.04 ± 2.20 per nucleus) was significantly greater as compared with colorectal adenomas (3.43 ± 0.88, P = 0.0000) and normal colorectal mucosa (1.25±0.09, P=0.0000). The number of bizarre Ag-NORs in colorectal carcinomas (61.39±22.38 in one hundred nuclei) was significantly higher than that in colorectal adenomas (20.74126.61, P = 0.0000) and normal colorectal mucosa (0,P= 0.0000).The number of small-sized (<1μm) and large-sized (>2μm) Ag-NORs in colorectal carcinomas (483.76±242.89 and 78.08±23.06 in one hundred nuclei) was significantly higher than that in colorectal adenomas (112.00±47.23 and 19.49±17.47, P = 0.0000 and 0.0000 respecti-vely) and normal colorectal mucosa (0 and 0.76 ± 1.16, P = 0.0000 and 0,0000 respectively). The distribution patterns including concentrated, dispersed, nucleolar and mixed type in carcinomas (23.64%, 53.20%, 9.30%, and 13.86%) differentiated significantly from those in colorectal adenomas (77.72%, 16.88%, 5.46% and 0.04%, all P<0.05) and in mormal colorectal mucosa (100%, 0, 0 and 0, all P<0.05). These results suggest that the four quantitative parameters of Ag-NORs might be useful for the differential diagnosis of carcinoma from adenoma of the colon.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
1993年第3期207-212,共6页
Journal of Sun Yat-Sen University:Medical Sciences
基金
中华医学基金会资助
