摘要
目的:介绍雪旺氏细胞体外培养的一种新方法;方法:先用IV型胶原酶和胰蛋白酶消化出生3天的SD鼠臂层神经和坐骨神经组织块后再将组织块贴壁培养。结果:雪旺氏细胞从组织块中“爬出”速度较直接组织贴壁培养方法快,且纯度较直接组织贴壁培养方法和酶组织块消化培养方法高。结论:先用酶消化组织块,后再将组织块贴壁培养雪旺氏细胞的方法简便可行,值得进一步推广。
Objective To introduce a new way for culturing Schwann cell in Vitro. Methods The sciatic and brachial plexus nerve of three-day-old SD mice was treated with collagenase type IV and trypsin to remove the desmocyte on the Schwann cell, then explanted in the culture disk. Results Schwann cell grew faster from the treated nerve than the nerve without treating,the purer Schwann cell was obtained from the treated nerve. Conclusion It is a simple, availability, worth spreading way to obtain the Schwann cell by the upper.
出处
《中国美容医学》
CAS
2004年第6期658-659,共2页
Chinese Journal of Aesthetic Medicine
基金
广东省科学技术研究项目(B2001065)
