摘要
Caco 2细胞来源于人类结肠腺癌细胞 ,当其在生长达到融合后细胞可自发形成极性 ,表达出成熟肠道上皮细胞的某些形态和功能特征 ,其细胞单层模型是目前最为成熟的体外模拟肠道上皮细胞摄取和转运营养物质和外来化学物的模型 ,被广泛地应用于药理学和毒理学研究。本文介绍了Caco 2细胞单层模型的培养条件、细胞特性及常用功能指标 ,以及其在对外源化学物代谢、摄取、转运和消化道毒理学方面的应用。
Caco-2 cells derived from a human colon adenocarcinoma, spontaneously differentiate after reaching confluence in culture, exhibiting several morphological and functional characteristics of mature enterocytes. Caco-2 cell monolayers represents a well-established model for the study of intestinal transport of nutrients and xenobiotics, and is wildly used in pharmacology and toxicology. In this review the cell culture conditions, characteristics and functional parameters of model were introduced. The application of Caco-2 cell monolayers in studying mechanism, uptake, transport and gastrointestinal toxicity of xenobiotics were also explained.
出处
《卫生研究》
CAS
CSCD
北大核心
2004年第6期756-759,共4页
Journal of Hygiene Research
基金
国际铜业协会 (ICA)资助项目 (No .TPT0 542E)
