摘要
目的 :探讨糖尿病 ( DM)大鼠肾组织 p38丝裂原活化蛋白激酶 ( MAPK)及其下游转录因子 c AMP反应元件结合蛋白 ( CREB)的表达特征及与肾小球肥大、细胞外基质积聚的关系。方法 :70只雄性 Wistar大鼠 ,行右肾切除术 2周后 ,随机分为两组 :右肾切除对照组 ( CN)和 DM组。 DM组经腹腔注射链脲佐菌素( STZ,6 5 mg/kg)诱发 DM。 CN组注射等量枸橼酸缓冲液。两组分别于注射后第 1、2、4、8和 12周各取 6只大鼠 ,收集 2 4 h尿液 ,测定尿蛋白 ( Upro)、肌酐 ( UCr) ;股动脉放血分离血清 ,测血糖 ( Glu)、血肌酐 ( SCr)、尿素氮( BU N) ,并计算肌酐清除率 ( CCr) ;免疫组化法检测肾皮质磷酸化 p38MAPK( P p38MAPK)及其磷酸化CREB( P CREB)的表达特征 ,并检测转化生长因子 β1 ( TGFβ1 )、纤维粘连蛋白 ( FN)及层粘连蛋白 ( L N)的表达 ,应用图像分析系统进行定量分析。流式细胞术检测 P p38MAPK和 P CREB蛋白的表达。结果 :与 CN组比较 ,DM组 P p38MAPK在第 1、2、4和 8周升高 ( P均 <0 .0 1) ,第 12周降至正常。DM组 P CREB在第2、4和 8周增高 ( P均 <0 .0 1) ,在 12周时降至正常。 TGFβ1 、FN、L N分别于第 2、4周开始升高。结论 :肾小球p38MAPK及其下游转录因子 CREB活性在早期糖尿病肾病大鼠肾组织中升高 。
Objective: To investigate the expression of the p38 mitogenactivated protein kinase (p38 MAPK) and cAMPresponse element binding protein (CREB) of kidney in experimental diabetes mellitus rats, and the relationship with glomerular hypertrophy and extracellular matrix(ECM) accumulation. Methods : Seventy male Wistar rats with nephrectomy of right kidney of 2 weeks were randomly divided in two groups: control group and diabetic group. Diabetic models were established by injecting intraperitoneally streptozocin (STZ, 65 mg/kg) and the control group was injected citric acidbuffered liquid. Six rats of each group were killed at 1, 2, 4, 8 and 12 weeks after injecting STZ respectively. Urine of 24 hours was collected, protein and creatinine in urine(Upro, UCr), serum glucose(Glu), serum creatinine(SCr), blood urea nitrogen(BUN) were determined, and creatinine clearance was calculated. Immunohistochemistry was used to analyze activation of phosphorylatingp38 MAPK(Pp38 MAPK) and phosphorylatingCREB(PCREB) in renal cortex, the expressions of transferase growth factorβ 1(TGFβ 1), fibronectin(FN), laminin(LN) in glomeruli were also analysed by computer imagepattern analysis system. Flow cytometry was used to detect the expression of Pp38 MAPK and PCREB. Results: The expressions of TGFβ 1, FN and LN in diabetic rats were increased in 2 and 4 weeks(all P <0 01). Compared with control group, expression of Pp38 MAPK in diabetic rats was significantly increased in 1, 2, 4, 8 weeks(all P <0 01), then decreased to normal in 12 weeks. PCREB was increased in 2, 4, 8 weeks in diabetic rats(all P <0 01), and decrease to normal in 12 weeks. Conclusion: Glomerular p38 MAPK and CREB activities are increased in early diabetic mellitus in rats. This activation of p38 MAPK pathway in diabetic glomeruli may, in part, plays a role in the pathogenesis of early glomerular hypertrophy and ECM accumulation.
出处
《中国中西医结合急救杂志》
CAS
2004年第6期372-376,共5页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
河北省自然科学基金资助项目 (3 0 2 5 0 0 )
