摘要
目的 :测定黄连及其制剂中小檗碱的含量。方法 :反相高效液相色谱法 ,HypersilODSC18柱 (2 5 0mm× 4 .6mm ,10 μm) ;流动相 :乙腈 (含 0 .0 2 5mol·L-1磷酸二氢钾与 0 .0 2 5mol·L-1庚烷磺酸钠的溶液 ) 水相 (含 0 .2 %三乙胺 ,用磷酸调节pH 3.0 ) (40∶6 0 ) ;检测波长为 35 0nm。结果 :盐酸小檗碱在 4～ 2 0 0mg·L-1浓度范围内 ,峰面积与其浓度呈良好的线性关系 ;片剂中盐酸小檗碱的平均回收率为 10 0 .4 % ,RSD为 0 .6 1%。结论 :方法简便 ,快速 ,准确。
OBJECTIVE To determine of berberine in Rhizoma Coptidis and its preparation.METHODS HYPERSIL ODS C 18 column 250 mm × 4.6 mm , 10 μm ,(Dalian Elite) was used the mobile phase consisted of acetonitrile (containing 0.025 mol ·L -1 phosphate and 0.025 mol ·L -1 sodium water solution (containing 0.25% trithylamine,pH was adjusted to 3.0 with phosphoric acid)(40∶60);detection wavelength was 350 nm .RESULTS The calibration curve was linear in the range of 4- 200 mg ·L -1 .The average recovery was 100.4% (RSD= 0.61% ) for tablets.CONCLUSION This method is found to be simple, and accurate for the separation of berberine in Rhizoma Coptidis and its preparation.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2004年第12期738-739,共2页
Chinese Journal of Hospital Pharmacy
